Non-volume-loaded heart provides a more relevant heterotopic transplantation model
Abstract Background We aimed to compare two techniques of heterotopic heart transplantation in rats. Non-volume-loaded (NL) and volume-loaded (VL) models were tested for their physiologic and immunologic properties to assess their suitability for transplant studies. Methods Syngeneic heterotopic hea...
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Published in | Transplant immunology Vol. 23; no. 1; pp. 65 - 70 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
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Elsevier B.V
01.05.2010
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Abstract | Abstract Background We aimed to compare two techniques of heterotopic heart transplantation in rats. Non-volume-loaded (NL) and volume-loaded (VL) models were tested for their physiologic and immunologic properties to assess their suitability for transplant studies. Methods Syngeneic heterotopic heart transplants were performed according to the techniques previously described by Ono (NL) and Yokoyama (VL). Grafts were followed over 90 days with sequential echocardiography. Ex-vivo Langendorff perfusion was used to gain functional data. Allogeneic heart transplants were done to determine whether chronic allograft vasculopathy (CAV) develops at a different pace in both transplant models. Results The ischemic time during surgery was significantly longer using the VL model ( p < 0.001). The LV diameter of NL hearts decreased over time while that of the VL model significantly increased ( p = 0.004 on POD 90). Mean LV developed pressure and (d P / d t )max were significantly higher with the NL model (61.1 ± 8.5 mmHg and 4261.7 ± 419.6 mmHg/s) than with VL hearts (19.9 ± 16.5 mmHg; p = 0.011 and 924.8 ± 605.6 mmHg/s; p < 0.001). The mean weight of NL hearts (0.45 ± 0.03 g) was significantly less than that of VL hearts (1.21 ± 0.16 g, p < 0.001). Histology of syngeneic NL grafts showed healthy, but partly atrophic myocardium, whereas the LV myocardium of VL hearts showed dilation and scarring typical for chronic ischemic injury. Heart allografts similarly developed CAV with luminal narrowing of 37.2 ± 16.6% (NL) and 34.4 ± 21.4% (VL), respectively by POD 90 ( p = 0.807). Conclusions Since the coronary arteries in the VL model get perfused with partly deoxygenated blood, the myocardium suffers from chronic ischemic injury. We recommend using the NL model in preclinical transplant studies. |
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AbstractList | We aimed to compare two techniques of heterotopic heart transplantation in rats. Non-volume-loaded (NL) and volume-loaded (VL) models were tested for their physiologic and immunologic properties to assess their suitability for transplant studies. Methods: Syngeneic heterotopic heart transplants were performed according to the techniques previously described by Ono (NL) and Yokoyama (VL). Grafts were followed over 90 days with sequential echocardiography. Ex-vivo Langendorff perfusion was used to gain functional data. Allogeneic heart transplants were done to determine whether chronic allograft vasculopathy (CAV) develops at a different pace in both transplant models. Results: The ischemic time during surgery was significantly longer using the VL model (p < 0.001). The LV diameter of NL hearts decreased over time while that of the VL model significantly increased (p = 0.004 on POD 90). Mean LV developed pressure and (dP / dt)max were significantly higher with the NL model (61.1 +/- 8.5 mmHg and 4261.7 +/- 419.6 mmHg/s) than with VL hearts (19.9 +/- 16.5 mmHg; p = 0.011 and 924.8 +/- 605.6 mmHg/s; p < 0.001). The mean weight of NL hearts (0.45 +/- 0.03 g) was significantly less than that of VL hearts (1.21 +/- 0.16 g, p < 0.001). Histology of syngeneic NL grafts showed healthy, but partly atrophic myocardium, whereas the LV myocardium of VL hearts showed dilation and scarring typical for chronic ischemic injury. Heart allografts similarly developed CAV with luminal narrowing of 37.2 +/- 16.6% (NL) and 34.4 +/- 21.4% (VL), respectively by POD 90 (p = 0.807). Conclusions: Since the coronary arteries in the VL model get perfused with partly deoxygenated blood, the myocardium suffers from chronic ischemic injury. We recommend using the NL model in preclinical transplant studies. We aimed to compare two techniques of heterotopic heart transplantation in rats. Non-volume-loaded (NL) and volume-loaded (VL) models were tested for their physiologic and immunologic properties to assess their suitability for transplant studies. Syngeneic heterotopic heart transplants were performed according to the techniques previously described by Ono (NL) and Yokoyama (VL). Grafts were followed over 90 days with sequential echocardiography. Ex-vivo Langendorff perfusion was used to gain functional data. Allogeneic heart transplants were done to determine whether chronic allograft vasculopathy (CAV) develops at a different pace in both transplant models. The ischemic time during surgery was significantly longer using the VL model ( p < 0.001). The LV diameter of NL hearts decreased over time while that of the VL model significantly increased ( p = 0.004 on POD 90). Mean LV developed pressure and (d P / d t)max were significantly higher with the NL model (61.1 ± 8.5 mmHg and 4261.7 ± 419.6 mmHg/s) than with VL hearts (19.9 ± 16.5 mmHg; p = 0.011 and 924.8 ± 605.6 mmHg/s; p < 0.001). The mean weight of NL hearts (0.45 ± 0.03 g) was significantly less than that of VL hearts (1.21 ± 0.16 g, p < 0.001). Histology of syngeneic NL grafts showed healthy, but partly atrophic myocardium, whereas the LV myocardium of VL hearts showed dilation and scarring typical for chronic ischemic injury. Heart allografts similarly developed CAV with luminal narrowing of 37.2 ± 16.6% (NL) and 34.4 ± 21.4% (VL), respectively by POD 90 ( p = 0.807). Since the coronary arteries in the VL model get perfused with partly deoxygenated blood, the myocardium suffers from chronic ischemic injury. We recommend using the NL model in preclinical transplant studies. We aimed to compare two techniques of heterotopic heart transplantation in rats. Non-volume-loaded (NL) and volume-loaded (VL) models were tested for their physiologic and immunologic properties to assess their suitability for transplant studies. Syngeneic heterotopic heart transplants were performed according to the techniques previously described by Ono (NL) and Yokoyama (VL). Grafts were followed over 90 days with sequential echocardiography. Ex-vivo Langendorff perfusion was used to gain functional data. Allogeneic heart transplants were done to determine whether chronic allograft vasculopathy (CAV) develops at a different pace in both transplant models. The ischemic time during surgery was significantly longer using the VL model (p<0.001). The LV diameter of NL hearts decreased over time while that of the VL model significantly increased (p=0.004 on POD 90). Mean LV developed pressure and (dP/dt)max were significantly higher with the NL model (61.1+/-8.5 mmHg and 4261.7+/-419.6 mmHg/s) than with VL hearts (19.9+/-16.5 mmHg; p=0.011 and 924.8+/-605.6 mmHg/s; p<0.001). The mean weight of NL hearts (0.45+/-0.03 g) was significantly less than that of VL hearts (1.21+/-0.16 g, p<0.001). Histology of syngeneic NL grafts showed healthy, but partly atrophic myocardium, whereas the LV myocardium of VL hearts showed dilation and scarring typical for chronic ischemic injury. Heart allografts similarly developed CAV with luminal narrowing of 37.2+/-16.6% (NL) and 34.4+/-21.4% (VL), respectively by POD 90 (p=0.807). Since the coronary arteries in the VL model get perfused with partly deoxygenated blood, the myocardium suffers from chronic ischemic injury. We recommend using the NL model in preclinical transplant studies. BACKGROUNDWe aimed to compare two techniques of heterotopic heart transplantation in rats. Non-volume-loaded (NL) and volume-loaded (VL) models were tested for their physiologic and immunologic properties to assess their suitability for transplant studies. METHODSSyngeneic heterotopic heart transplants were performed according to the techniques previously described by Ono (NL) and Yokoyama (VL). Grafts were followed over 90 days with sequential echocardiography. Ex-vivo Langendorff perfusion was used to gain functional data. Allogeneic heart transplants were done to determine whether chronic allograft vasculopathy (CAV) develops at a different pace in both transplant models. RESULTSThe ischemic time during surgery was significantly longer using the VL model (p<0.001). The LV diameter of NL hearts decreased over time while that of the VL model significantly increased (p=0.004 on POD 90). Mean LV developed pressure and (dP/dt)max were significantly higher with the NL model (61.1+/-8.5 mmHg and 4261.7+/-419.6 mmHg/s) than with VL hearts (19.9+/-16.5 mmHg; p=0.011 and 924.8+/-605.6 mmHg/s; p<0.001). The mean weight of NL hearts (0.45+/-0.03 g) was significantly less than that of VL hearts (1.21+/-0.16 g, p<0.001). Histology of syngeneic NL grafts showed healthy, but partly atrophic myocardium, whereas the LV myocardium of VL hearts showed dilation and scarring typical for chronic ischemic injury. Heart allografts similarly developed CAV with luminal narrowing of 37.2+/-16.6% (NL) and 34.4+/-21.4% (VL), respectively by POD 90 (p=0.807). CONCLUSIONSSince the coronary arteries in the VL model get perfused with partly deoxygenated blood, the myocardium suffers from chronic ischemic injury. We recommend using the NL model in preclinical transplant studies. Abstract Background We aimed to compare two techniques of heterotopic heart transplantation in rats. Non-volume-loaded (NL) and volume-loaded (VL) models were tested for their physiologic and immunologic properties to assess their suitability for transplant studies. Methods Syngeneic heterotopic heart transplants were performed according to the techniques previously described by Ono (NL) and Yokoyama (VL). Grafts were followed over 90 days with sequential echocardiography. Ex-vivo Langendorff perfusion was used to gain functional data. Allogeneic heart transplants were done to determine whether chronic allograft vasculopathy (CAV) develops at a different pace in both transplant models. Results The ischemic time during surgery was significantly longer using the VL model ( p < 0.001). The LV diameter of NL hearts decreased over time while that of the VL model significantly increased ( p = 0.004 on POD 90). Mean LV developed pressure and (d P / d t )max were significantly higher with the NL model (61.1 ± 8.5 mmHg and 4261.7 ± 419.6 mmHg/s) than with VL hearts (19.9 ± 16.5 mmHg; p = 0.011 and 924.8 ± 605.6 mmHg/s; p < 0.001). The mean weight of NL hearts (0.45 ± 0.03 g) was significantly less than that of VL hearts (1.21 ± 0.16 g, p < 0.001). Histology of syngeneic NL grafts showed healthy, but partly atrophic myocardium, whereas the LV myocardium of VL hearts showed dilation and scarring typical for chronic ischemic injury. Heart allografts similarly developed CAV with luminal narrowing of 37.2 ± 16.6% (NL) and 34.4 ± 21.4% (VL), respectively by POD 90 ( p = 0.807). Conclusions Since the coronary arteries in the VL model get perfused with partly deoxygenated blood, the myocardium suffers from chronic ischemic injury. We recommend using the NL model in preclinical transplant studies. |
Author | Robbins, Robert C Bartos, Jason Mochly-Rosen, Daria Churchill, Eric Deuse, Tobias Tang-Quan, Karis R Schäfer, Hansjörg Reichenspurner, Hermann Schrepfer, Sonja |
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CitedBy_id | crossref_primary_10_1152_ajpheart_00218_2013 crossref_primary_10_1016_j_healun_2014_11_015 crossref_primary_10_1016_j_jss_2013_01_018 crossref_primary_10_1016_j_transproceed_2013_03_036 crossref_primary_10_3389_fcvm_2016_00034 |
Cites_doi | 10.1016/S1053-2498(99)00062-5 10.1016/j.echo.2005.10.005 10.1016/j.healun.2006.05.002 10.1161/01.CIR.100.1.67 10.1016/j.hfc.2007.02.007 10.1016/S0022-5223(19)42744-X 10.1016/S1053-2498(01)00401-6 |
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Keywords | Yokoyama Ono–Lindsey Chronic ischemia Immunology Rat Transplant vasculopathy Non-volume-loaded heart transplant model Heterotopic heart transplantation Volume-loaded heart transplant model |
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References_xml | – volume: 14 start-page: 7 year: 1995 ident: bib5 article-title: Proposal of a working left heart model with a heterotopic transplantation technique in rats publication-title: J Heart Lung Transplant contributor: fullname: Mohri – volume: 49 start-page: 8 year: 2007 ident: bib9 article-title: A novel platform device for rodent echocardiography publication-title: ILAR e-Journal contributor: fullname: Sista – volume: 100 start-page: 67 year: 1999 ident: bib10 article-title: Rapamycin reverses chronic graft vascular disease in a novel cardiac allograft model publication-title: Circulation contributor: fullname: Hoyt – volume: 18 start-page: 10 year: 1999 ident: bib6 article-title: A simple new model of physiologically working heterotopic rat heart transplantation provides hemodynamic performance equivalent to that of an orthotopic heart publication-title: J Heart Lung Transplant contributor: fullname: Kohl – volume: 25 start-page: 869 year: 2006 ident: bib1 article-title: Registry of the International Society for Heart and Lung Transplantation: twenty-third official adult heart transplantation report—2006 publication-title: J Heart Lung Transplant contributor: fullname: Boucek – volume: 21 start-page: 4 year: 2002 ident: bib7 article-title: Preferential involvement of larger vessels in a rat model of diabetes-induced graft vasculopathy publication-title: J Heart Lung Transplant contributor: fullname: Wen – volume: 89 start-page: 13 year: 1964 ident: bib3 article-title: A technique for heart transplantation in the rat publication-title: Surgery contributor: fullname: De Witt – volume: 18 start-page: 24 year: 2005 ident: bib8 article-title: Recommendations for chamber quantification: a report from the American Society of Echocardiography's Guidelines and Standards Committee and the Chamber Quantification Writing Group, Developed in Conjunction with the European Association of Echocardiography, a branch of the European Society of Cardiology publication-title: J Am Soc Echocardiogr contributor: fullname: Devereux – volume: 3 start-page: 87 year: 2007 ident: bib2 article-title: Cardiac allograft vasculopathy: an update publication-title: Heart Fail Clin contributor: fullname: Starling – volume: 57 start-page: 5 year: 1969 ident: bib4 article-title: Improved technique of heart transplantation in rats publication-title: J Thorac Cardiovasc Surg contributor: fullname: Lindsey – volume: 18 start-page: 10 issue: 10 year: 1999 ident: 10.1016/j.trim.2010.04.005_bib6 article-title: A simple new model of physiologically working heterotopic rat heart transplantation provides hemodynamic performance equivalent to that of an orthotopic heart publication-title: J Heart Lung Transplant doi: 10.1016/S1053-2498(99)00062-5 contributor: fullname: Asfour – volume: 18 start-page: 24 issue: 12 year: 2005 ident: 10.1016/j.trim.2010.04.005_bib8 publication-title: J Am Soc Echocardiogr doi: 10.1016/j.echo.2005.10.005 contributor: fullname: Lang – volume: 49 start-page: 8 year: 2007 ident: 10.1016/j.trim.2010.04.005_bib9 article-title: A novel platform device for rodent echocardiography publication-title: ILAR e-Journal contributor: fullname: Kutschka – volume: 25 start-page: 869 issue: 8 year: 2006 ident: 10.1016/j.trim.2010.04.005_bib1 article-title: Registry of the International Society for Heart and Lung Transplantation: twenty-third official adult heart transplantation report—2006 publication-title: J Heart Lung Transplant doi: 10.1016/j.healun.2006.05.002 contributor: fullname: Taylor – volume: 89 start-page: 13 year: 1964 ident: 10.1016/j.trim.2010.04.005_bib3 article-title: A technique for heart transplantation in the rat publication-title: Surgery contributor: fullname: Abbott – volume: 100 start-page: 67 issue: 1 year: 1999 ident: 10.1016/j.trim.2010.04.005_bib10 article-title: Rapamycin reverses chronic graft vascular disease in a novel cardiac allograft model publication-title: Circulation doi: 10.1161/01.CIR.100.1.67 contributor: fullname: Poston – volume: 3 start-page: 87 issue: 1 year: 2007 ident: 10.1016/j.trim.2010.04.005_bib2 article-title: Cardiac allograft vasculopathy: an update publication-title: Heart Fail Clin doi: 10.1016/j.hfc.2007.02.007 contributor: fullname: Sipahi – volume: 57 start-page: 5 issue: 2 year: 1969 ident: 10.1016/j.trim.2010.04.005_bib4 article-title: Improved technique of heart transplantation in rats publication-title: J Thorac Cardiovasc Surg doi: 10.1016/S0022-5223(19)42744-X contributor: fullname: Ono – volume: 14 start-page: 7 issue: 4 year: 1995 ident: 10.1016/j.trim.2010.04.005_bib5 article-title: Proposal of a working left heart model with a heterotopic transplantation technique in rats publication-title: J Heart Lung Transplant contributor: fullname: Yokoyama – volume: 21 start-page: 4 issue: 9 year: 2002 ident: 10.1016/j.trim.2010.04.005_bib7 article-title: Preferential involvement of larger vessels in a rat model of diabetes-induced graft vasculopathy publication-title: J Heart Lung Transplant doi: 10.1016/S1053-2498(01)00401-6 contributor: fullname: Cantin |
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Snippet | Abstract Background We aimed to compare two techniques of heterotopic heart transplantation in rats. Non-volume-loaded (NL) and volume-loaded (VL) models were... We aimed to compare two techniques of heterotopic heart transplantation in rats. Non-volume-loaded (NL) and volume-loaded (VL) models were tested for their... BACKGROUNDWe aimed to compare two techniques of heterotopic heart transplantation in rats. Non-volume-loaded (NL) and volume-loaded (VL) models were tested for... |
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SubjectTerms | Allergy and Immunology Animals Aorta, Abdominal - transplantation Aorta, Thoracic - diagnostic imaging Cardiac Volume - physiology Chronic ischemia Diastole Echocardiography Heart - physiopathology Heart Transplantation - methods Heterotopic heart transplantation Immunology Male Models, Animal Myocardium - pathology Non-volume-loaded heart transplant model Ono–Lindsey Rat Rats Transplant vasculopathy Transplantation, Heterotopic Vena Cava, Inferior - pathology Volume-loaded heart transplant model Yokoyama |
Title | Non-volume-loaded heart provides a more relevant heterotopic transplantation model |
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