Characterisation of canine CD34+/CD45 diminished cells by colony-forming unit assay and transcriptome analysis
Haematopoietic stem and progenitor cells (HSPCs) are used for transplantation to reconstruct the haematopoietic pathways in humans receiving severe chemotherapy. However, the characteristics of canine HSPCs, such as specific surface antigens and gene expression profiles, are still unclear. This stud...
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| Published in | Frontiers in veterinary science Vol. 9; p. 936623 |
|---|---|
| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English |
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Frontiers Media S.A
12.09.2022
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| Abstract | Haematopoietic stem and progenitor cells (HSPCs) are used for transplantation to reconstruct the haematopoietic pathways in humans receiving severe chemotherapy. However, the characteristics of canine HSPCs, such as specific surface antigens and gene expression profiles, are still unclear. This study aimed to characterise the haematopoietic ability and gene expression profiles of canine bone marrow HSPCs in healthy dogs. In this study, the CD34 positive (CD34+) cells were defined as classical HSPCs, CD34+/CD45 diminished (CD45
dim
) cells as more enriched HSPCs, and whole viable cells as controls. Haematopoietic abilities and gene expression profiles were evaluated using a colony-forming unit assay and RNA-sequencing analysis. Canine CD34+/CD45
dim
cells exhibited a significantly higher haematopoietic colony formation ability and expressed more similarity in the gene expression profiles to human and mouse HSPCs than those of the other cell fractions. Furthermore, the canine CD34+/CD45
dim
cells expressed candidate cell surface antigens necessary to define the canine haematopoietic hierarchy roadmap. These results indicate that the canine CD34+/CD45
dim
cells express the HSPC characteristics more than the other cell fractions, thereby suggesting that these cells have the potential to be used for studying haematopoietic stem cells in dogs. |
|---|---|
| AbstractList | Haematopoietic stem and progenitor cells (HSPCs) are used for transplantation to reconstruct the haematopoietic pathways in humans receiving severe chemotherapy. However, the characteristics of canine HSPCs, such as specific surface antigens and gene expression profiles, are still unclear. This study aimed to characterise the haematopoietic ability and gene expression profiles of canine bone marrow HSPCs in healthy dogs. In this study, the CD34 positive (CD34+) cells were defined as classical HSPCs, CD34+/CD45 diminished (CD45dim) cells as more enriched HSPCs, and whole viable cells as controls. Haematopoietic abilities and gene expression profiles were evaluated using a colony-forming unit assay and RNA-sequencing analysis. Canine CD34+/CD45dim cells exhibited a significantly higher haematopoietic colony formation ability and expressed more similarity in the gene expression profiles to human and mouse HSPCs than those of the other cell fractions. Furthermore, the canine CD34+/CD45dim cells expressed candidate cell surface antigens necessary to define the canine haematopoietic hierarchy roadmap. These results indicate that the canine CD34+/CD45dim cells express the HSPC characteristics more than the other cell fractions, thereby suggesting that these cells have the potential to be used for studying haematopoietic stem cells in dogs.Haematopoietic stem and progenitor cells (HSPCs) are used for transplantation to reconstruct the haematopoietic pathways in humans receiving severe chemotherapy. However, the characteristics of canine HSPCs, such as specific surface antigens and gene expression profiles, are still unclear. This study aimed to characterise the haematopoietic ability and gene expression profiles of canine bone marrow HSPCs in healthy dogs. In this study, the CD34 positive (CD34+) cells were defined as classical HSPCs, CD34+/CD45 diminished (CD45dim) cells as more enriched HSPCs, and whole viable cells as controls. Haematopoietic abilities and gene expression profiles were evaluated using a colony-forming unit assay and RNA-sequencing analysis. Canine CD34+/CD45dim cells exhibited a significantly higher haematopoietic colony formation ability and expressed more similarity in the gene expression profiles to human and mouse HSPCs than those of the other cell fractions. Furthermore, the canine CD34+/CD45dim cells expressed candidate cell surface antigens necessary to define the canine haematopoietic hierarchy roadmap. These results indicate that the canine CD34+/CD45dim cells express the HSPC characteristics more than the other cell fractions, thereby suggesting that these cells have the potential to be used for studying haematopoietic stem cells in dogs. Haematopoietic stem and progenitor cells (HSPCs) are used for transplantation to reconstruct the haematopoietic pathways in humans receiving severe chemotherapy. However, the characteristics of canine HSPCs, such as specific surface antigens and gene expression profiles, are still unclear. This study aimed to characterise the haematopoietic ability and gene expression profiles of canine bone marrow HSPCs in healthy dogs. In this study, the CD34 positive (CD34+) cells were defined as classical HSPCs, CD34+/CD45 diminished (CD45dim) cells as more enriched HSPCs, and whole viable cells as controls. Haematopoietic abilities and gene expression profiles were evaluated using a colony-forming unit assay and RNA-sequencing analysis. Canine CD34+/CD45dim cells exhibited a significantly higher haematopoietic colony formation ability and expressed more similarity in the gene expression profiles to human and mouse HSPCs than those of the other cell fractions. Furthermore, the canine CD34+/CD45dim cells expressed candidate cell surface antigens necessary to define the canine haematopoietic hierarchy roadmap. These results indicate that the canine CD34+/CD45dim cells express the HSPC characteristics more than the other cell fractions, thereby suggesting that these cells have the potential to be used for studying haematopoietic stem cells in dogs. Haematopoietic stem and progenitor cells (HSPCs) are used for transplantation to reconstruct the haematopoietic pathways in humans receiving severe chemotherapy. However, the characteristics of canine HSPCs, such as specific surface antigens and gene expression profiles, are still unclear. This study aimed to characterise the haematopoietic ability and gene expression profiles of canine bone marrow HSPCs in healthy dogs. In this study, the CD34 positive (CD34+) cells were defined as classical HSPCs, CD34+/CD45 diminished (CD45 dim ) cells as more enriched HSPCs, and whole viable cells as controls. Haematopoietic abilities and gene expression profiles were evaluated using a colony-forming unit assay and RNA-sequencing analysis. Canine CD34+/CD45 dim cells exhibited a significantly higher haematopoietic colony formation ability and expressed more similarity in the gene expression profiles to human and mouse HSPCs than those of the other cell fractions. Furthermore, the canine CD34+/CD45 dim cells expressed candidate cell surface antigens necessary to define the canine haematopoietic hierarchy roadmap. These results indicate that the canine CD34+/CD45 dim cells express the HSPC characteristics more than the other cell fractions, thereby suggesting that these cells have the potential to be used for studying haematopoietic stem cells in dogs. |
| Author | Nitta, Suguru Horie, Ryo Hisasue, Masaharu Ayabe, Taro Neo, Sakurako Yamada, Yoko Matsumoto, Yuki Kawamoto, Kosuke Kikuchi, Kaoruko |
| AuthorAffiliation | 2 Research and Development Section, Anicom Specialty Medical Institute Inc. , Yokohama , Japan 1 Laboratory of Small Animal Internal Medicine, School of Veterinary Medicine, Azabu University , Sagamihara , Japan 3 Laboratory of Clinical Diagnosis, School of Veterinary Medicine, Azabu University , Sagamihara , Japan |
| AuthorAffiliation_xml | – name: 3 Laboratory of Clinical Diagnosis, School of Veterinary Medicine, Azabu University , Sagamihara , Japan – name: 1 Laboratory of Small Animal Internal Medicine, School of Veterinary Medicine, Azabu University , Sagamihara , Japan – name: 2 Research and Development Section, Anicom Specialty Medical Institute Inc. , Yokohama , Japan |
| Author_xml | – sequence: 1 givenname: Taro surname: Ayabe fullname: Ayabe, Taro – sequence: 2 givenname: Masaharu surname: Hisasue fullname: Hisasue, Masaharu – sequence: 3 givenname: Yoko surname: Yamada fullname: Yamada, Yoko – sequence: 4 givenname: Suguru surname: Nitta fullname: Nitta, Suguru – sequence: 5 givenname: Kaoruko surname: Kikuchi fullname: Kikuchi, Kaoruko – sequence: 6 givenname: Sakurako surname: Neo fullname: Neo, Sakurako – sequence: 7 givenname: Yuki surname: Matsumoto fullname: Matsumoto, Yuki – sequence: 8 givenname: Ryo surname: Horie fullname: Horie, Ryo – sequence: 9 givenname: Kosuke surname: Kawamoto fullname: Kawamoto, Kosuke |
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| Copyright | Copyright © 2022 Ayabe, Hisasue, Yamada, Nitta, Kikuchi, Neo, Matsumoto, Horie and Kawamoto. Copyright © 2022 Ayabe, Hisasue, Yamada, Nitta, Kikuchi, Neo, Matsumoto, Horie and Kawamoto. 2022 Ayabe, Hisasue, Yamada, Nitta, Kikuchi, Neo, Matsumoto, Horie and Kawamoto |
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| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Andrea Pires Dos Santos, Purdue University, United States; Carlos Alberto Antunes Viegas, University of Trás-os-Montes and Alto Douro, Portugal This article was submitted to Veterinary Experimental and Diagnostic Pathology, a section of the journal Frontiers in Veterinary Science Edited by: Marxa L. Figueiredo, Purdue University, United States |
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