Noise-induced hearing loss vulnerability in type III intermediate filament peripherin gene knockout mice

• Published in: Frontiers in neurology Vol. 13; p. 962227
• Main Authors: Cederholm, Jennie M. E., Parley, Kristina E., Perera, Chamini J., von Jonquieres, Georg, Pinyon, Jeremy L., Julien, Jean-Pierre, Ryugo, David K., Ryan, Allen F., Housley, Gary D.
• Format: Journal Article
• Language: English
• Published: Frontiers Media S.A 26.09.2022
• Subjects: cochlea; contralateral suppression; distortion product otoacoustic emission (DPOAE); medial olivocochlear (MOC) efferents; Neurology; type II spiral ganglion neurons
• ISSN: 1664-2295; 1664-2295
• DOI: 10.3389/fneur.2022.962227

In the post-natal mouse cochlea, type II spiral ganglion neurons (SGNs) innervating the electromotile outer hair cells (OHCs) of the ‘cochlear amplifier' selectively express the type III intermediate filament peripherin gene ( Prph) . Immunolabeling showed that Prph knockout (KO) mice exhibited disruption of this (outer spiral bundle) afferent innervation, while the radial fiber (type I SGN) innervation of the inner hair cells (~95% of the SGN population) was retained. Functionality of the medial olivocochlear (MOC) efferent innervation of the OHCs was confirmed in the Prph KO, based on suppression of distortion product otoacoustic emissions (DPOAEs) via direct electrical stimulation. However, “contralateral suppression” of the MOC reflex neural circuit, evident as a rapid reduction in cubic DPOAE when noise is presented to the opposite ear in wildtype mice, was substantially disrupted in the Prph KO. Auditory brainstem response (ABR) measurements demonstrated that hearing sensitivity (thresholds and growth-functions) were indistinguishable between wildtype and Prph KO mice. Despite this comparability in sound transduction and strength of the afferent signal to the central auditory pathways, high-intensity, broadband noise exposure (108 dB SPL, 1 h) produced permanent high frequency hearing loss (24–32 kHz) in Prph KO mice but not the wildtype mice, consistent with the attenuated contralateral suppression of the Prph KO. These data support the postulate that auditory neurons expressing Prph contribute to the sensory arm of the otoprotective MOC feedback circuit.