The essential role of cobalt in the inhibition of the cytosolic isozyme of 3-deoxy- d- arabino-heptulosonate-7-phosphate synthase from Nicotiana silvestris by glyphosate

• Published in: Archives of biochemistry and biophysics Vol. 260; no. 1; pp. 85 - 93
• Main Authors: Ganson, Robert J., Jensen, Roy A.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 1988; Elsevier
• Subjects: 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase; 3-Phosphoshikimate 1-Carboxyvinyltransferase; activity; Alkyl and Aryl Transferases; Analytical, structural and metabolic biochemistry; Biological and medical sciences; cobalt; Cobalt - pharmacology; Cytosol - enzymology; Dose-Response Relationship, Drug; enzyme activity; Enzymes and enzyme inhibitors; Fundamental and applied biological sciences. Psychology; Glycine - analogs & derivatives; Glycine - pharmacology; Glyphosate; inhibition; Isoenzymes - antagonists & inhibitors; isozymes; Kinetics; ligases; Miscellaneous; Nicotiana - enzymology; Nicotiana silvestris; Nicotiana sylvestris; Plants, Toxic; role; Spectrophotometry; Substrate Specificity; Sugar Phosphates - metabolism; Transferases - antagonists & inhibitors

The prime molecular target of glyphosate ( N-[phosphonomethyl]glycine), a potent herbicidal and antimicrobial agent, is known to be the shikimate-pathway enzyme, 5- enol-pyruvylshikimate-3-phosphate synthase. Inhibition by glyphosate of an earlier pathway enzyme that is located in the cytosol of hig...