Two chromatographic schemes for protein purification involving the biotin/avidin interaction under native conditions

• Published in: Journal of Chromatography A Vol. 1621; p. 461051
• Main Authors: Raducanu, Vlad-Stefan, Tehseen, Muhammad, Shirbini, Afnan, Raducanu, Daniela-Violeta, Hamdan, Samir M.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 21.06.2020
• Subjects: Avidin; AviTag; Biotin; biotinylation; BirA; buffers; Chromatography; Escherichia coli; humans; ligases; Macrobac; plasmids; proliferating cell nuclear antigen; proteinases; solubility; BirA; Macrobac; Biotin; AviTag; Avidin; Chromatography

•Protein purification under native conditions employing the biotin/avidin interaction.•Single expression vector for BirA and AviTag-tagged proteins.•Efficient in vivo biotinylation using BirA enzyme.•An optimized release system from the biotin/avidin interaction.•Efficient coupling of a biotin-tagge...