Smilax China L. Rhizome Extract Inhibits Nuclear Factor-κ B and Induces Apoptosis in Ovarian Cancer Cells

• Published in: Chinese journal of integrative medicine Vol. 21; no. 12; pp. 907 - 915
• Main Author: 胡丽玲 陈东生 王燕燕 秦铀 黄璞 于丽秀 廖婧 华小黎
• Format: Journal Article
• Language: English
• Published: Beijing Chinese Association of Traditional and Western Medicine 01.12.2015
• Subjects: Apoptosis - drug effects; caspase-3; Cell Line, Tumor; Cell Survival - drug effects; Female; Humans; Medicine; Medicine & Public Health; NF-kappa B - antagonists & inhibitors; Original Article; Ovarian Neoplasms - drug therapy; Ovarian Neoplasms - pathology; Plant Extracts - pharmacology; Smilax; 卵巢癌细胞; 提取物; 核因子-κB; 根茎; 细胞凋亡; 菝葜; 诱导; nuclear factor-κB; G; A2780 cells; apoptosis; M arrest; L. rhizome; chemosensitivity; G2/M arrest; Smilax china L. rhizome
• ISSN: 1672-0415; 1993-0402; 1993-0402
• DOI: 10.1007/s11655-014-1788-9

Objective： To study the antitumor effects and associated mechanisms of extract of the Smilax china L. rhizome （SCR） on ovarian cancer cells. Methods： Ovarian cancer cells A2780 were treated with different concentrations of SCR extract （SCRE）, and compared with controls. Effects on cell growth were evaluated by cell counting kit-8 （CCK-8） assay; proliferation effects by EdU incorporation assay; cell cycle by propidium iodide staining; apoptosis by annexin V-fluorescein isothiocyanate/propidium iodide; cellular distribution of nuclear factor- κ B （NF-κ B） by immunofluorescence; protein levels of NF- κB, caspase-3, poly-adenosine diphosphate （ADP）-ribose polymerase （PARP）, Bcl-2-associated X protein （Bax）, cellular inhibitor of apoptosis （clAP）-1, anti-X-linked inhibitor of apoptosis protein （XlAP）, B-cell lymphoma-extra large （BcI-XL）, B-cell lymphoma-2 （Bcl-2） and AKT by Western blotting; and effects of SCRE combined with cisplatin or adriamycin on A2780 cells by CCK-8 assay. Results： SCRE suppressed A2780 cell proliferation in a dose-dependent manner （P〈0.05, /=〈0.01）, arrested cells in GJM phase and induced apoptosis by activating caspase-3, PARP and Bax. SCRE treatment also correlated with inhibition of NF-κ B and downregulation of Bcl-2, Bcl-XL, clAP-l, XlAP and AKT. SCRE can promote chemosensitivity to cisplatin and adriamycin in A2780 cells （P〈0.01）. Conclusion： SCR effectively inhibits NF- κ B, induces apoptosis and reduces chemoresistance to cisplatin and adriamycin in ovarian cancer cells, which might be its molecular basis for treating ovarian cancer.