End invasion of peptide nucleic acids (PNAs) with mixed-base composition into linear DNA duplexes

• Published in: Nucleic acids research Vol. 33; no. 17; p. e146
• Main Authors: Smolina, Irina V., Demidov, Vadim V., Soldatenkov, Viatcheslav A., Chasovskikh, Sergey G., Frank-Kamenetskii, Maxim D.
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.01.2005; Oxford Publishing Limited (England)
• Subjects: DNA - analysis; DNA - chemistry; DNA - ultrastructure; DNA Primers - chemistry; Electrophoretic Mobility Shift Assay; Fluorescent Dyes; Methods Online; Microscopy, Atomic Force; Oligonucleotide Probes - chemistry; Peptide Nucleic Acids - chemistry; Peptide Nucleic Acids - ultrastructure; Polymerase Chain Reaction
• DOI: 10.1093/nar/gni151
• ISSN: 0305-1048

Peptide nucleic acid (PNA) is a synthetic DNA mimic with valuable properties and a rapidly growing scope of applications. With the exception of recently introduced pseudocomplementary PNAs, binding of common PNA oligomers to target sites located inside linear double-stranded DNAs (dsDNAs) is essentially restricted to homopurine–homopyrimidine sequence motifs, which significantly hampers some of the PNA applications. Here, we suggest an approach to bypass this limitation of common PNAs. We demonstrate that PNA with mixed composition of ordinary nucleobases is capable of sequence-specific targeting of complementary dsDNA sites if they are located at the very termini of DNA duplex. We then show that such targeting makes it possible to perform capturing of designated dsDNA fragments via the DNA-bound biotinylated PNA as well as to signal the presence of a specific dsDNA sequence, in the case a PNA beacon is employed. We also examine the PNA–DNA conjugate and prove that it can initiate the primer-extension reaction starting from the duplex DNA termini when a DNA polymerase with the strand-displacement ability is used. We thus conclude that recognition of duplex DNA by mixed-base PNAs via the end invasion has a promising potential for site-specific and sequence-unrestricted DNA manipulation and detection.