Acclimation to low temperature by microsomal membranes from tomato cell cultures

• Published in: Plant physiology (Bethesda) Vol. 77; no. 1; pp. 74 - 78
• Main Authors: DuPont, F.M, Mudd, J.B
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.01.1985
• Subjects: Absorption. Translocation of ions and substances. Permeability; ADAPTACION FISIOLOGICA; ADAPTATION; Adenosine triphosphatases; Agronomy. Soil science and plant productions; Biological and medical sciences; Cell culture techniques; Cell membranes; Cell physiology; CELL STRUCTURE; Cooling; CULTIVO DE TEJIDOS; CULTURE DE TISSUS; CYTOPLASMIC ORGANELLES; Economic plant physiology; Effects of physical and chemical agents; ESTRUCTURA CELULAR; Fluorescence; Fundamental and applied biological sciences. Psychology; HIDROLASAS; HYDROLASE; HYDROLASES; Low temperature; Lycopersicon esculentum; membrane vesicles; microsomes; Molecular and cellular biology; Nutrition. Photosynthesis. Respiration. Metabolism; ORGANITE CELLULAIRE; ORGANULOS CITOPLASMATICOS; Plant cells; Plants; proton transport; Protons; STRUCTURE CELLULAIRE; temperature; TEMPERATURES; TISSUE CULTURE; TOMATE; TOMATOES; Tonoplast; Vesicle; Cell culture; Vegetals; Cold; ATPase; Fluorescence; Microsome; Suspension culture; Dicotyledones; Angiospermae; Lycopersicon esculentum; Membrane; Spermatophyta; Solanaceae; Adaptation; Proton transport

Sealed vesicles were prepared from microsomal membranes from cell suspension cultures of tomato (Lycopersicon esculentum Mill cv VF36). ATP-dependent proton transport activity by the vesicles was measured as quenching of fluorescence of acridine orange. Measurements of proton transport were correlat...