Glyceraldehyde-3-phosphate dehydrogenase is a major protein associated with the plasma membrane of retinal photoreceptor outer segments

• Published in: The Journal of biological chemistry Vol. 265; no. 22; pp. 13308 - 13313
• Main Authors: SHU-CHAN HSU, MOLDAY, R. S
• Format: Journal Article
• Language: English
• Published: Bethesda, MD American Society for Biochemistry and Molecular Biology 05.08.1990
• Subjects: Amino Acid Sequence; Animals; Biological and medical sciences; Cattle; Cell Membrane - enzymology; Cell physiology; Chymotrypsin - pharmacology; Erythrocyte Membrane - enzymology; Fundamental and applied biological sciences. Psychology; Glyceraldehyde-3-Phosphate Dehydrogenases - genetics; Glyceraldehyde-3-Phosphate Dehydrogenases - isolation & purification; Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism; Humans; Kinetics; Molecular and cellular biology; Molecular Sequence Data; Molecular Weight; Photoreceptor Cells - enzymology; photoreceptors; plasma membranes; Protein Binding; retina; Rod Cell Outer Segment - enzymology; Sequence Homology, Nucleic Acid; Trypsin - pharmacology; Vision, photoreception; Purification; Bovine; Enzyme; Rod; Retina; Vertebrata; Mammalia; Enzymatic activity; Affinity chromatography; Glyceraldehyde-phosphate dehydrogenase; Plasma membrane; Artiodactyla; Ungulata

A major 38-kDa protein associated with bovine rod outer segment plasma membranes, but not disk membranes, has been identified as glyceraldehyde-3-phosphate dehydrogenase on the basis of its N-terminal sequence and specific enzyme activity. This enzyme was extracted from lysed rod outer segments or i...