Effects of Ginsenoside Rg-1 on the Proliferation and Osteogenic Differentiation of Human Periodontal Ligament Stem Cells

• Published in: Chinese journal of integrative medicine Vol. 21; no. 9; pp. 676 - 681
• Main Author: 殷丽华 程文晓 秦子顺 孙可墨 钟梅 王家奎 高维岳 余占海
• Format: Journal Article
• Language: English
• Published: Beijing Chinese Association of Traditional and Western Medicine 01.09.2015
• Subjects: Adolescent; Alkaline Phosphatase - metabolism; Biomarkers - metabolism; Calcification, Physiologic - drug effects; Cell Differentiation - drug effects; Cell Proliferation - drug effects; Cell Separation; Cell Shape - drug effects; Cells, Cultured; Flow Cytometry; Ginsenosides - pharmacology; Humans; Medicine; Medicine & Public Health; Original Article; Osteoblasts - drug effects; Osteoblasts - metabolism; Osteogenesis - drug effects; Osteogenesis - genetics; Periodontal Ligament - cytology; Real-Time Polymerase Chain Reaction; Stem Cells - cytology; Stem Cells - drug effects; Stem Cells - enzymology; Time Factors; Young Adult; 人参皂苷Rg1; 人牙周膜干细胞; 增殖分化; 成骨分化; 最佳浓度; 碱性磷酸酶活性; 聚合酶链反应; 转录因子; human periodontal ligament stem cell; Chinese medicine; proliferation; ginsenoside Rg-1; osteogenic differentiation
• ISSN: 1672-0415; 1993-0402; 1993-0402
• DOI: 10.1007/s11655-014-1856-9

Objective： TO evaluate the effects of ginsenoside Rg-1 on the proliferation and osteogenic differentiation of human periodontal ligament stem cells （hPDLSCs） and to explore the possible application on the alveolar bone regeneration. Methods： To determine the optimum concentration, the effects of ginsenoside Rg-1 ranging from 10 to 100 μmol/L were evaluated by 3-（4,5）-dimethylthiahiazo（-z-yl）-3,5-di-phenytetrazoliumromide, alkaline phosphatase activity and calcium deposition. Expressions of runt-related transcription factor 2, collagen alpha-2（I） chain, osteopontin, osteocalcin protein were examined using real-time polymerase chain reaction. Results： Compared with the control group, a certain concentration （10 μmol/L） of the Rg-1 solution significantly enhanced the proliferation and osteogenic differentiation of hPDLSCs （P〈0.05）. However, concentrations that exceeds 100 μmol/L led to cytotoxicity whereas concentrations below 10 nmol/L showed no significant effect as compared with the control. Conclusion： Ginsenoside Rg-1 can enhance the proliferation and osteogenic differentiation of hPDLSCs at an optimal concentration of 10 μmol/L.