Modelling Förster resonance energy transfer (FRET) using anisotropy resolved multi-dimensional emission spectroscopy (ARMES)

• Published in: Biochimica et biophysica acta. General subjects Vol. 1865; no. 2; p. 129770
• Main Authors: Gordon, Fiona, Elcoroaristizabal, Saioa, Ryder, Alan G.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.02.2021
• Subjects: Algorithms; Anilino Naphthalenesulfonates - chemistry; Anisotropy; Chemometrics; energy transfer; Fluorescence; Fluorescence Resonance Energy Transfer - methods; fluorescent dyes; Fluorescent Dyes - chemistry; Förster resonance energy transfer; human serum albumin; Humans; Modelling; Models, Molecular; multivariate analysis; Protein; Serum Albumin, Human - chemistry; spectroscopy; sulfonates; tryptophan; tyrosine; HH; VV; Förster resonance energy transfer; Fluorescence; IFE; HV; MDF; pEEM; ARMES; Trp; HSA; Modelling; FRET; ANS, 1; TSFS; EEM; Tyr; pTSFS; Protein; LOR; VH; Anisotropy; PARAFAC; MCR; Chemometrics

Förster Resonance Energy Transfer (FRET) is widely used to study the structure and dynamics of biomolecular systems and also causes the non-linear fluorescence response observed in multi-fluorophore proteins. Accurate FRET analysis, in terms of measuring changes in donor and acceptor spectra and ene...