Correlation of Der p 2 T-cell responses with clinical characteristics of children allergic to house dust mite
Abstract Background An understanding of the mechanisms responsible for the development and maintenance of allergic inflammation and their clinical implications is needed to develop specific and successful treatment for allergy. Objectives To characterize in vitro T-cell responses to Der p 2, one of...
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Published in | Annals of allergy, asthma, & immunology Vol. 109; no. 6; pp. 442 - 447 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
New York, NY
Elsevier Inc
01.12.2012
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | Abstract Background An understanding of the mechanisms responsible for the development and maintenance of allergic inflammation and their clinical implications is needed to develop specific and successful treatment for allergy. Objectives To characterize in vitro T-cell responses to Der p 2, one of the major allergens of house dust mite (HDM), and investigate potential correlations between clinical and laboratory parameters. Methods Forty-two patients monosensitized to HDM and 10 age-matched, healthy children were studied. Dendritic cells pulsed with Der p 2 were used to stimulate autologous CD14− cells. Der p 2–specific T-cell activation markers, proliferation, and cytokine production profiles were examined. Results Der p 2–specific T-cell activation markers, proliferation, and TH 2 cytokine production were significantly higher in HDM patients compared with healthy controls. Moreover, a significant correlation between proliferation and TH 2 cytokine production was observed. Within the allergic group, skin reaction to HDM was significantly stronger in patients with a Der p 2–specific T-cell response. Levels of HDM-specific IgE directly correlated with interleukin 5 and interleukin 13 levels and with skin prick test results and, ultimately, with the patient's family history of allergy. Furthermore, the presence of atopic march correlated with T-cell proliferation. Conclusion We found that, in HDM patients, Der p 2–specific TH 2 responses, promoted by autologous dendritic cells in vitro, correlate with clinical parameters. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1081-1206 1534-4436 |
DOI: | 10.1016/j.anai.2012.09.001 |