Study of the Cys-His bridge electron transfer pathway in a copper-containing nitrite reductase by site-directed mutagenesis, spectroscopic, and computational methods - Cristaldi, Julio C.; Gómez, María C.; González, Pablo J.; Ferroni, Felix M.; Dalosto, Sergio D.; Rizzi, Alberto C.; Rivas, María G.; Brondino, Carlos D. (Biochimica et biophysica acta, 2018)
Keywords: catalytic activity; Copper; Denitrification; electron paramagnetic resonance spectroscopy; electron transfer; Electron transfer pathway; Ensifer meliloti; enzyme activity; Enzyme mechanism; hydrogen bonding; monitoring; nitric oxide; Nitrite reductase; nitrites; oxidation; paraquat; point mutation; Sinorhizobium meliloti 2011; site-directed mutagenesis; spectral analysis; ultraviolet-visible spectroscopy; Rs; wt SmNir; Af; Nitrite reductase; MV; QM/MM; Sinorhizobium meliloti 2011; SmPaz; H171D; Electron transfer pathway; Enzyme mechanism; Ax; Denitrification; C172D; Copper