Real-time functional analysis of Hv1 channel in neutrophils: a new approach from zebrafish model

• Published in: American journal of physiology. Regulatory, integrative and comparative physiology Vol. 316; no. 6; pp. R819 - R831
• Main Authors: Ratanayotha, Adisorn, Kawai, Takafumi, Okamura, Yasushi
• Format: Journal Article
• Language: English
• Published: United States American Physiological Society 01.06.2019
• Subjects: Animals; Animals, Genetically Modified; Bacterial infections; Calcium; Calcium Signaling; Danio rerio; Embryos; Functional analysis; Immune response; Immune system; Immunity, Innate; In vivo methods and tests; Infections; Innate immunity; Ion Channel Gating; Ion Channels - deficiency; Ion Channels - genetics; Ion Channels - metabolism; Larvae; Leukocytes (neutrophilic); Mammals; Membrane Potentials; Neutrophils; Neutrophils - immunology; Neutrophils - metabolism; Phagocytosis; Phagosomes; Phagosomes - immunology; Phagosomes - metabolism; Physiology; Protons; Reactive oxygen species; Reactive Oxygen Species - metabolism; Real time; Survival analysis; Time Factors; Vertebrates; Zebrafish; Zebrafish - embryology; Zebrafish - genetics; Zebrafish - immunology; Zebrafish - metabolism; Zebrafish Proteins - deficiency; Zebrafish Proteins - genetics; Zebrafish Proteins - metabolism; proton channel; phagocytosis; in vivo model; zebrafish; reactive oxygen species

Voltage-gated proton channel (Hv1) has been studied in various immune cells, including neutrophils. However, most studies have taken an in vitro approach using isolated cells or primary cultured cells of mammals; therefore, limited evidence is available on the function of Hv1 in a physiological cont...