Acridine orange interaction with DNA: Effect of ionic strength

• Published in: Biochimica et biophysica acta. General subjects Vol. 1861; no. 4; pp. 900 - 909
• Main Authors: Amado, André M., Pazin, Wallance M., Ito, Amando S., Kuzmin, Vladimir A., Borissevitch, Iouri E.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2017
• Subjects: absorption; acridine orange; Acridine Orange - chemistry; DNA; DNA - chemistry; fluorescence; ionic strength; light scattering; Osmolar Concentration; photosensitizing agents; sodium chloride; Sodium Chloride - chemistry; spectral analysis; Spectrometry, Fluorescence - methods; spectroscopy

The study of acridine orange (AO) spectral characteristics and the quenching of its singlet and triplet excited states by TEMPO radical at its binding to DNA in the function of the DNA concentration and in the absence and presence of NaCl is reported. The study was performed using steady-state and t...