Production of lipases by Rhizopus oligosporous by solid-state fermentation

The present investigation describes the production of lipase by solid-state fermentation. Ten mould cultures were screened for the production of lipases. Rhizopus oligosporous GCBR-3 supported maximum production of lipases (48.0 U/g substrate) under optimum environmental and cultural conditions such...

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Bibliographic Details
Published inProcess biochemistry (1991) Vol. 37; no. 6; pp. 637 - 641
Main Authors ul-Haq, Ikram, Idrees, Shumaila, Rajoka, M.Ibrahim
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 01.01.2002
Subjects
Baggase
Lipases
Meals
Oils
Rhizopus
Sugarcane
Baggase
Oils
Meals
Lipases
Sugarcane
Rhizopus
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Summary:The present investigation describes the production of lipase by solid-state fermentation. Ten mould cultures were screened for the production of lipases. Rhizopus oligosporous GCBR-3 supported maximum production of lipases (48.0 U/g substrate) under optimum environmental and cultural conditions such as selection of efficient organism from the available microorganisms, selection of almond meal as the best substrate among different substrates, inoculum size, extractants/diluents and incubation period. The organism secreted the enzyme extracellularly. To study the kinetics of enzyme production, the organism was grown under optimum growth conditions for enzyme production. The lipase value supported by the organism and calculated as units per gram substrate per hour was greater than the values reported by other workers. This organism can be exploited for large-scale production of enzyme for commercial purposes.
ISSN:1359-5113
1873-3298
DOI:10.1016/S0032-9592(01)00252-7