An ex vivo model to induce early fibrosis-like changes in human precision-cut lung slices

• Published in: American journal of physiology. Lung cellular and molecular physiology Vol. 312; no. 6; pp. L896 - L902
• Main Authors: Alsafadi, Hani N., Staab-Weijnitz, Claudia A., Lehmann, Mareike, Lindner, Michael, Peschel, Britta, Königshoff, Melanie, Wagner, Darcy E.
• Format: Journal Article
• Language: English
• Published: United States American Physiological Society 01.06.2017
• Subjects: Aged; Alveolar Epithelial Cells - metabolism; Alveolar Epithelial Cells - pathology; Alveoli; Biomarkers - metabolism; Collagen (type I); Collagen Type I - metabolism; Connective tissue growth factor; Enzyme-linked immunosorbent assay; Epithelium; Explants; Extracellular matrix; Extracellular Matrix - metabolism; Female; Fibrosis; Gene expression; Growth factors; Histology; Humans; Idiopathic Pulmonary Fibrosis - pathology; Immunofluorescence; Inflammation Mediators - metabolism; Lung - pathology; Lung diseases; Lungs; Lysophosphatidic acid; Male; Matrilysin; Matrix; Models, Biological; Molecular modelling; Molecules; Morbidity; Platelet-derived growth factor; Protein C; Proteins; Pulmonary fibrosis; Scars; Surfactant protein C; Tissue Survival; Tissues; Transforming growth factor; Transforming growth factor-b; Tumor necrosis factor-α; Up-Regulation; precision-cut lung slices; fibrosis; idiopathic pulmonary fibrosis; ex vivo; disease model

Idiopathic pulmonary fibrosis (IPF) is a devastating chronic interstitial lung disease (ILD) characterized by lung tissue scarring and high morbidity. Lung epithelial injury, myofibroblast activation, and deranged repair are believed to be key processes involved in disease onset and progression, but...