Leptin induces the generation of procoagulant, tissue factor bearing microparticles by human peripheral blood mononuclear cells

• Published in: Biochimica et biophysica acta Vol. 1860; no. 6; pp. 1354 - 1361
• Main Authors: Petrini, Silvia, Neri, Tommaso, Lombardi, Stefania, Cordazzo, Cinzia, Balìa, Cristina, Scalise, Valentina, Paggiaro, Pierluigi, Pedrinelli, Roberto, Celi, Alessandro
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.06.2016
• Subjects: apoptosis; blood coagulation; Blood Coagulation Factors - biosynthesis; body mass index; calcium; Calcium - metabolism; calmodulin; Calmodulin - physiology; Cell-Derived Microparticles - drug effects; Cell-Derived Microparticles - physiology; Cells, Cultured; Extracellular Signal-Regulated MAP Kinases - metabolism; flow cytometry; fluorescence; Humans; Leptin; Leptin - pharmacology; Leukocytes, Mononuclear - metabolism; Microparticles; mitogen-activated protein kinase; Monocytes; mononuclear leukocytes; Obesity; phosphatidylserines; phospholipase C; risk; risk reduction; Thromboplastin - biosynthesis; Thrombosis; Tissue factor; Type C Phospholipases - physiology; PBS; Obesity; SSc; MP; PS; [Ca2+]i; JNK; PLC; Tissue factor; CFSE; MTT; MAPK; PBMC; Thrombosis; Microparticles; TF; FITC; Monocytes; PE; Leptin; FSc; ERK

Obesity is linked to increased thrombotic risk. Circulating leptin concentration correlates with body mass index. Microparticles are small (.05–1μm) vesicles shed by activated and apoptotic cells, involved in numerous pathophysiologically relevant phenomena including blood coagulation and thrombosis...