Application of BMP-2/FGF-2 gene–activated scaffolds for dental pulp capping

• Published in: Clinical oral investigations Vol. 24; no. 12; pp. 4427 - 4437
• Main Authors: Chakka, Leela Raghava Jaidev, Vislisel, Jered, Vidal, Cristina de Mattos Pimenta, Biz, Michelle Tillmann, K. Salem, Aliasger, Cavalcanti, Bruno Neves
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.12.2020; Springer Nature B.V
• Subjects: Bone growth; Bone morphogenetic protein 2; Cell culture; Cell Differentiation; Cell growth; Cell Proliferation; Cells, Cultured; Collagen; Connective tissues; Cytotoxicity; Dental Pulp; Dental Pulp Capping; Dentistry; Electrophoretic mobility; Fibroblast growth factor 2; Fibroblast Growth Factor 2 - genetics; Gene therapy; Humans; Light scattering; Medicine; Mineralization; Original Article; Polyethyleneimine; Root canals; Stem cells; Tissue Engineering; Tissue Scaffolds; Transfection; Transplantation; Zeta potential; Stem cells; Pulp capping tissue engineering; DPSC transfection; Endodontics; Scaffold; Gene-activated collagen scaffolds; Dental pulp
• ISSN: 1432-6981; 1436-3771
• DOI: 10.1007/s00784-020-03308-2

Objectives To evaluate the effect of non-viral gene therapy on human dental pulp stem cells (DPSCs) in an in vitro and an ex vivo model. Materials and methods Nanoplexes comprising polyethyleneimine (PEI) and plasmid DNA (pDNA) encoding for fibroblast growth factor-2 (p FGF-2 ) and bone morphogenic protein-2 (p BMP-2 ) were cultured with DPSCs to evaluate cytotoxicity, protein expression, and mineralization activity. Collagen scaffolds loaded with these nanoplexes or mineral trioxide aggregate (MTA) were utilized in an ex vivo tooth culture model to assess pulp response, over a period of 14 days. All nanoplex formulations were characterized for size and zeta potential by measuring dynamic light scattering and electrophoretic mobility, respectively. Results DPSCs treated with the nanoplexes showed increased cell proliferation and enhanced expression of BMP-2 and FGF-2 proteins. Collagen scaffolds containing PEI-p BMP-2 and/or p FGF-2 nanoplexes significantly increased cell proliferation, BMP-2 and FGF-2 expression, and mineralization when compared to MTA. Ex vivo histology showed a well-preserved pulp and healthy tissue in both the MTA and scaffold groups. Connective tissue in contact with the scaffold was dense and homogeneous, with some cells present in contact and within the scaffold. Conclusion Transfection of DPSCs with p BMP-2 /p FGF-2 nanoplexes resulted in increased expression of BMP-2 and FGF-2, enhanced proliferation, and mineralization properties compared to MTA. These findings were supported by the ex vivo observations. Clinical relevance This biological approach in pulp capping brings new insights into the effective management of engineered pulp tissues, mainly those generated by the transplantation of DPSCs in empty root canals.