Structure and dynamics of Trichoderma harzianum Cel7B suggest molecular architecture adaptations required for a wide spectrum of activities on plant cell wall polysaccharides

• Published in: Biochimica et biophysica acta. General subjects Vol. 1863; no. 6; pp. 1015 - 1026
• Main Authors: Sonoda, Milton T., Godoy, Andre S., Pellegrini, Vanessa O.A., Kadowaki, Marco A.S., Nascimento, Alessandro S., Polikarpov, Igor
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.06.2019
• Subjects: active sites; Cell Wall - chemistry; cell walls; Cellulase; Cellulase - chemistry; endo-1,4-beta-glucanase; Fungal Proteins - chemistry; fungi; GH7; glycosides; glycosylation; hydrogen bonding; lignocellulose; molecular dynamics; Molecular Dynamics Simulation; Molecular dynamics simulations; Oligosaccharides - chemistry; oxygen; Plant Cells - chemistry; Protein crystallography; simulation models; Trichoderma - enzymology; Trichoderma harzianum; X-radiation; xylooligosaccharides; Trichoderma harzianum; Molecular dynamics simulations; Cellulase; Protein crystallography; GH7

Cellulases from glycoside hydrolase family 7 (GH7) play crucial roles in plant lignocellulose deconstruction by fungi, but structural information available for GH7 fungal endoglucanases is limited when compared to the number of known sequences in the family. Here, we report the X-ray structure of th...