On‐chip microfluidic buffer swap of biological samples in‐line with downstream dielectrophoresis

• Published in: Electrophoresis Vol. 43; no. 12; pp. 1275 - 1282
• Main Authors: Huang, Xuhai, Torres‐Castro, Karina, Varhue, Walter, Rane, Aditya, Rasin, Ahmed, Swami, Nathan S.
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.06.2022
• Subjects: Biological properties; Buffers; Cell Separation - methods; cell viability; centrifugation; Dielectrophoresis; Diffusion rate; Dilution; Electric Conductivity; electrophoresis; Electrophoresis - methods; Electrophysiology; Flow velocity; hydrodynamics; Ion diffusion; Media; Microfluidic Analytical Techniques - methods; Microfluidics; Oligonucleotide Array Sequence Analysis; Physiology; sample preparation; separation; Serpentine; Straight channels; tangential flows; dielectrophoresis; separation; microfluidics; tangential flows; sample preparation

Microfluidic cell enrichment by dielectrophoresis, based on biophysical and electrophysiology phenotypes, requires that cells be resuspended from their physiological media into a lower conductivity buffer for enhancing force fields and enabling the dielectric contrast needed for separation. To ensur...