In situ digestion of alcohol-fixed cells for quantitative proteomics

Currently, the bottom-up approach, in which proteins are digested by enzymes such as trypsin prior to mass spectrometry, is the mainstream approach in mass spectrometer-based proteomics. In this approach, the enzymatic digestion process strongly affects the reproducibility of protein identification...

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Bibliographic Details
Published inJournal of biochemistry (Tokyo) Vol. 173; no. 4; pp. 243 - 254
Main Authors Hatano, Atsushi, Takami, Tomoyo, Matsumoto, Masaki
Format Journal Article
LanguageEnglish
Published England 01.04.2023
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