Ooplasmic injections of rabbit round spermatid nuclei or intact round spermatids from fresh, cryopreserved and cryostored samples

We compared the outcome of ooplasmic round spermatid nuclear injections (ROSNI) versus intact round spermatid injections (ROSI). Rabbit round spermatid nuclei and intact round spermatids were recovered and injected into rabbit oocytes (groups A and B, respectively). Fertilization, cleavage and embry...

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Published inHuman reproduction (Oxford) Vol. 14; no. 6; pp. 1506 - 1515
Main Authors Yamamoto, Yasuhisa, Sofikitis, Nikolaos, Miyagawa, Ikuo
Format Journal Article
LanguageEnglish
Published Oxford Oxford University Press 01.06.1999
Subjects
Animals
Biological and medical sciences
Cryopreservation
Cryoprotective Agents
cryostorage
Cryptorchidism
Cytoplasm
Dimethyl Sulfoxide
Embryonic and Fetal Development
Female
Fertilization in Vitro - methods
Fundamental and applied biological sciences. Psychology
Glucose
Glycerol
Male
Mammalian reproduction. General aspects
Microinjections
Nuclear Transfer Techniques
Oocytes - ultrastructure
Rabbits
Semen Preservation
spermatids
Spermatids - ultrastructure
Tromethamine
Vertebrates: reproduction
spermatids
cryostorage
cryptorchidism
rabbits
cryopreservation
Vertebrata
Mammalia
Cryopreservation
Intracytoplasmic sperm injection
Assisted procreation
Rabbit
Germinal cell
Lagomorpha
Oocyte
Spermatid
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Abstract We compared the outcome of ooplasmic round spermatid nuclear injections (ROSNI) versus intact round spermatid injections (ROSI). Rabbit round spermatid nuclei and intact round spermatids were recovered and injected into rabbit oocytes (groups A and B, respectively). Fertilization, cleavage and embryonic development rates were compared. In additional studies, five protocols for cryopreservation of round spermatids and two protocols for cryostorage of round spermatids were applied. The outcome of ROSNI techniques using frozen–thawed or cryostored–warmed round spermatids was evaluated. The cleavage rate and the overall morula plus blastocyst development rate were significantly larger in group A than group B. ROSNI procedures are superior to ROSI techniques in the rabbit. The largest fertilization, cleavage and embryonic development rates after ROSNI techniques using cryopreserved or cryostored round spermatids were demonstrated in groups of round spermatids in which a mixture of seminal plasma plus test yolk buffer was employed as an extender, and dimethyl sulphoxide plus a high concentration of glycerol served as cryoprotectants. It appears that the seminal plasma contains factors protecting round spermatids during cryopreservation or cryostorage, and/or the employment of two cryoprotectants has a beneficial role in the maintenance of round spermatid reproductive capacity.
AbstractList We compared the outcome of ooplasmic round spermatid nuclear injections (ROSNI) versus intact round spermatid injections (ROSI). Rabbit round spermatid nuclei and intact round spermatids were recovered and injected into rabbit oocytes (groups A and B, respectively). Fertilization, cleavage and embryonic development rates were compared. In additional studies, five protocols for cryopreservation of round spermatids and two protocols for cryostorage of round spermatids were applied. The outcome of ROSNI techniques using frozen-thawed or cryostored-warmed round spermatids was evaluated. The cleavage rate and the overall morula plus blastocyst development rate were significantly larger in group A than group B. ROSNI procedures are superior to ROSI techniques in the rabbit. The largest fertilization, cleavage and embryonic development rates after ROSNI techniques using cryopreserved or cryostored round spermatids were demonstrated in groups of round spermatids in which a mixture of seminal plasma plus test yolk buffer was employed as an extender, and dimethyl sulphoxide plus a high concentration of glycerol served as cryoprotectants. It appears that the seminal plasma contains factors protecting round spermatids during cryopreservation or cryostorage, and/or the employment of two cryoprotectants has a beneficial role in the maintenance of round spermatid reproductive capacity.
Author Miyagawa, Ikuo
Yamamoto, Yasuhisa
Sofikitis, Nikolaos
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Cites_doi 10.1007/BF02066177
10.1093/oxfordjournals.humrep.a019261
10.1016/S0015-0282(16)58048-0
10.1006/dbio.1994.1064
10.1083/jcb.69.3.539
10.1093/humrep/13.1.104
10.1007/BF02765754
10.1016/S0140-6736(05)79121-2
10.1093/humrep/13.2.255
10.1093/humrep/12.3.554
10.1095/biolreprod48.2.219
10.1017/S0967199400001234
10.1016/S0015-0282(98)00106-X
10.1159/000186678
10.1093/oxfordjournals.humrep.a019694
10.1093/oxfordjournals.humrep.a019693
10.1095/biolreprod55.4.895
10.1095/biolreprod53.4.855
10.1016/S0140-6736(05)61819-3
10.1093/humrep/13.10.2977
10.1095/biolreprod49.1.33
10.1042/bj0580618
10.1093/humrep/12.12.2713
10.1016/S0015-0282(16)58644-0
10.1006/dbio.1994.1256
10.1093/humrep/12.2.286
10.1095/biolreprod52.4.709
10.1242/dev.121.8.2397
10.1016/S0022-5347(01)66014-2
10.1073/pnas.91.16.7460
10.1007/BF02214138
10.1016/S0015-0282(16)56835-6
10.1016/0014-4827(84)90442-7
10.1038/nm0495-289
10.1056/NEJM199508243330819
10.1093/humrep/12.11.2422
10.1093/humrep/13.3.709
10.1093/humrep/12.6.1203
10.1126/science.168.3934.939
10.1093/oxfordjournals.humrep.a138426
10.1093/humupd/4.3.197
10.1016/0301-2115(95)02220-2
10.1016/S0015-0282(16)56894-0
10.1093/humrep/12.Suppl_2.81-a
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Issue 6
Keywords spermatids
cryostorage
cryptorchidism
rabbits
cryopreservation
Vertebrata
Mammalia
Cryopreservation
Intracytoplasmic sperm injection
Assisted procreation
Rabbit
Germinal cell
Lagomorpha
Oocyte
Spermatid
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References key 20170410032919_SOFIKITIS-ETAL-1995B
key 20170410032919_KAHRAMAN-ETAL-1998
key 20170410032919_ODONELL-ETAL-1996
key 20170410032919_SOFIKITIS-ETAL-1995A
key 20170410032919_MANSOUR-ETAL-1996
key 20170410032919_SOFIKITIS-AND-MIYAGAWA-1994B
key 20170410032919_YAMANAKA-ETAL-1997
key 20170410032919_SILBERT-AND-JOHNSON-1998
key 20170410032919_AMER-ETAL-1997
key 20170410032919_OGURA-ETAL-1994
key 20170410032919_SOFIKITIS-ETAL-1994C
key 20170410032919_OGURA-ETAL-1996
key 20170410032919_SOFIKITIS-ETAL-1994A
key 20170410032919_MAZIA-1984
key 20170410032919_SOFIKITIS-ETAL-1998C
key 20170410032919_SOFIKITIS-ETAL-1998A
key 20170410032919_SOFIKITIS-ETAL-1998B
key 20170410032919_TANAKA-ETAL-1996
key 20170410032919_SILBERT-ETAL-1997
key 20170410032919_HANNAY-1995
key 20170410032919_LI-ETAL-1993
key 20170410032919_TESARIK-ETAL-1998A
key 20170410032919_TESARIK-ETAL-1998B
key 20170410032919_BARAK-ETAL-1998
key 20170410032919_OGURA-ETAL-1993
key 20170410032919_SOFIKITIS-ETAL-1997B
key 20170410032919_SOFIKITIS-ETAL-1997A
key 20170410032919_ANTINORI-ETAL-1997B
key 20170410032919_OGURA-AND-YANAGIMACHI-1993
key 20170410032919_TESARIK-ETAL-1995
key 20170410032919_ANTINORI-ETAL-1997A
key 20170410032919_HYODO-ETAL-1991
key 20170410032919_GOTO-ETAL-1996
key 20170410032919_KIMURA-AND-YANAGIMACHI-1995A
key 20170410032919_KIMURA-AND-YANAGIMACHI-1995B
key 20170410032919_NAVARA-ETAL-1994
key 20170410032919_SHERMAN-1997
key 20170410032919_SOUSA-ETAL-1998
key 20170410032919_KIMURA-AND-YANAGIMACHI-1995C
key 20170410032919_MAZUR-1970
key 20170410032919_LOVELOCK-AND-POLGE-1954
key 20170410032919_LONGO-1976
key 20170410032919_KOFINAS-AND-ZAVOS-1992
key 20170410032919_SOFIKITIS-ETAL-1996A
key 20170410032919_SOFIKITIS-ETAL-1996B
key 20170410032919_VANDERZWALMEN-ETAL-1997
key 20170410032919_SCHATTEN-1994
key 20170410032919_VANDERZWALMEN-ETAL-1998
key 20170410032919_MENDOZA-AND-TESARIK-1996
key 20170410032919_EDWARDS-ETAL-1994
key 20170410032919_BARISIC-ETAL-1995
key 20170410032919_BARISIC-ETAL-1994
key 20170410032919_SASAGAWA-AND-YANAGIMACHI-1997
References_xml – ident: key 20170410032919_SOFIKITIS-AND-MIYAGAWA-1994B
– ident: key 20170410032919_OGURA-ETAL-1996
  doi: 10.1007/BF02066177
– ident: key 20170410032919_GOTO-ETAL-1996
  doi: 10.1093/oxfordjournals.humrep.a019261
– ident: key 20170410032919_SOFIKITIS-ETAL-1996B
  doi: 10.1016/S0015-0282(16)58048-0
– ident: key 20170410032919_MANSOUR-ETAL-1996
– ident: key 20170410032919_NAVARA-ETAL-1994
  doi: 10.1006/dbio.1994.1064
– ident: key 20170410032919_LONGO-1976
  doi: 10.1083/jcb.69.3.539
– ident: key 20170410032919_KAHRAMAN-ETAL-1998
  doi: 10.1093/humrep/13.1.104
– ident: key 20170410032919_YAMANAKA-ETAL-1997
  doi: 10.1007/BF02765754
– ident: key 20170410032919_SOFIKITIS-ETAL-1998C
  doi: 10.1016/S0140-6736(05)79121-2
– ident: key 20170410032919_SOUSA-ETAL-1998
  doi: 10.1093/humrep/13.2.255
– ident: key 20170410032919_ANTINORI-ETAL-1997B
  doi: 10.1093/humrep/12.3.554
– ident: key 20170410032919_OGURA-AND-YANAGIMACHI-1993
  doi: 10.1095/biolreprod48.2.219
– ident: key 20170410032919_OGURA-ETAL-1993
  doi: 10.1017/S0967199400001234
– ident: key 20170410032919_TANAKA-ETAL-1996
– ident: key 20170410032919_BARAK-ETAL-1998
  doi: 10.1016/S0015-0282(98)00106-X
– ident: key 20170410032919_HYODO-ETAL-1991
  doi: 10.1159/000186678
– ident: key 20170410032919_TESARIK-ETAL-1998A
  doi: 10.1093/oxfordjournals.humrep.a019694
– ident: key 20170410032919_SILBERT-AND-JOHNSON-1998
– ident: key 20170410032919_KOFINAS-AND-ZAVOS-1992
– ident: key 20170410032919_VANDERZWALMEN-ETAL-1998
  doi: 10.1093/oxfordjournals.humrep.a019693
– ident: key 20170410032919_SHERMAN-1997
– ident: key 20170410032919_ODONELL-ETAL-1996
  doi: 10.1095/biolreprod55.4.895
– ident: key 20170410032919_KIMURA-AND-YANAGIMACHI-1995B
  doi: 10.1095/biolreprod53.4.855
– ident: key 20170410032919_AMER-ETAL-1997
  doi: 10.1016/S0140-6736(05)61819-3
– ident: key 20170410032919_TESARIK-ETAL-1998B
  doi: 10.1093/humrep/13.10.2977
– ident: key 20170410032919_LI-ETAL-1993
  doi: 10.1095/biolreprod49.1.33
– ident: key 20170410032919_LOVELOCK-AND-POLGE-1954
  doi: 10.1042/bj0580618
– ident: key 20170410032919_SOFIKITIS-ETAL-1997A
  doi: 10.1093/humrep/12.12.2713
– ident: key 20170410032919_MENDOZA-AND-TESARIK-1996
  doi: 10.1016/S0015-0282(16)58644-0
– ident: key 20170410032919_SCHATTEN-1994
  doi: 10.1006/dbio.1994.1256
– ident: key 20170410032919_SOFIKITIS-ETAL-1995B
– ident: key 20170410032919_SASAGAWA-AND-YANAGIMACHI-1997
– ident: key 20170410032919_ANTINORI-ETAL-1997A
  doi: 10.1093/humrep/12.2.286
– ident: key 20170410032919_KIMURA-AND-YANAGIMACHI-1995C
  doi: 10.1095/biolreprod52.4.709
– ident: key 20170410032919_KIMURA-AND-YANAGIMACHI-1995A
  doi: 10.1242/dev.121.8.2397
– ident: key 20170410032919_SOFIKITIS-ETAL-1996A
  doi: 10.1016/S0022-5347(01)66014-2
– ident: key 20170410032919_OGURA-ETAL-1994
  doi: 10.1073/pnas.91.16.7460
– ident: key 20170410032919_SOFIKITIS-ETAL-1994A
  doi: 10.1007/BF02214138
– ident: key 20170410032919_BARISIC-ETAL-1994
  doi: 10.1016/S0015-0282(16)56835-6
– ident: key 20170410032919_MAZIA-1984
  doi: 10.1016/0014-4827(84)90442-7
– ident: key 20170410032919_HANNAY-1995
  doi: 10.1038/nm0495-289
– ident: key 20170410032919_TESARIK-ETAL-1995
  doi: 10.1056/NEJM199508243330819
– ident: key 20170410032919_SILBERT-ETAL-1997
  doi: 10.1093/humrep/12.11.2422
– ident: key 20170410032919_SOFIKITIS-ETAL-1998B
  doi: 10.1093/humrep/13.3.709
– ident: key 20170410032919_VANDERZWALMEN-ETAL-1997
  doi: 10.1093/humrep/12.6.1203
– ident: key 20170410032919_MAZUR-1970
  doi: 10.1126/science.168.3934.939
– ident: key 20170410032919_EDWARDS-ETAL-1994
  doi: 10.1093/oxfordjournals.humrep.a138426
– ident: key 20170410032919_SOFIKITIS-ETAL-1998A
  doi: 10.1093/humupd/4.3.197
– ident: key 20170410032919_SOFIKITIS-ETAL-1995A
– ident: key 20170410032919_BARISIC-ETAL-1995
  doi: 10.1016/0301-2115(95)02220-2
– ident: key 20170410032919_SOFIKITIS-ETAL-1994C
  doi: 10.1016/S0015-0282(16)56894-0
– ident: key 20170410032919_SOFIKITIS-ETAL-1997B
  doi: 10.1093/humrep/12.Suppl_2.81-a
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Snippet We compared the outcome of ooplasmic round spermatid nuclear injections (ROSNI) versus intact round spermatid injections (ROSI). Rabbit round spermatid nuclei...
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SubjectTerms Animals
Biological and medical sciences
Cryopreservation
Cryoprotective Agents
cryostorage
Cryptorchidism
Cytoplasm
Dimethyl Sulfoxide
Embryonic and Fetal Development
Female
Fertilization in Vitro - methods
Fundamental and applied biological sciences. Psychology
Glucose
Glycerol
Male
Mammalian reproduction. General aspects
Microinjections
Nuclear Transfer Techniques
Oocytes - ultrastructure
Rabbits
Semen Preservation
spermatids
Spermatids - ultrastructure
Tromethamine
Vertebrates: reproduction
Title Ooplasmic injections of rabbit round spermatid nuclei or intact round spermatids from fresh, cryopreserved and cryostored samples
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https://www.ncbi.nlm.nih.gov/pubmed/10357967
https://search.proquest.com/docview/69808489
Volume 14
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