A novel flow cytometric method to assess inflammasome formation

• Published in: The Journal of immunology (1950) Vol. 194; no. 1; pp. 455 - 462
• Main Authors: Sester, David P, Thygesen, Sara J, Sagulenko, Vitaliya, Vajjhala, Parimala R, Cridland, Jasmyn A, Vitak, Nazarii, Chen, Kaiwen W, Osborne, Geoffrey W, Schroder, Kate, Stacey, Katryn J
• Format: Journal Article
• Language: English
• Published: United States 01.01.2015
• Subjects: Animals; Apoptosis - immunology; Apoptosis Regulatory Proteins - genetics; Apoptosis Regulatory Proteins - immunology; Bone Marrow Cells - immunology; CARD Signaling Adaptor Proteins - immunology; Caspase 1 - genetics; Cell Line; Flow Cytometry - methods; HEK293 Cells; Humans; Inflammasomes - analysis; Inflammasomes - immunology; Inflammation Mediators - immunology; Macrophages - immunology; Mice; Mice, Knockout

Inflammasomes are large protein complexes induced by a wide range of microbial, stress, and environmental stimuli that function to induce cell death and inflammatory cytokine processing. Formation of an inflammasome involves dramatic relocalization of the inflammasome adapter protein apoptosis-assoc...