Interaction between protein kinase C-dependent and G protein-dependent pathways in the regulation of natural killer cell granule exocytosis

• Published in: The Journal of biological chemistry Vol. 267; no. 33; pp. 23957 - 23962
• Main Authors: TING, A. T, SCHOON, R. A, ABRAHAM, R. T, LEIBSON, P. J
• Format: Journal Article
• Language: English
• Published: Bethesda, MD American Society for Biochemistry and Molecular Biology 25.11.1992
• Subjects: Alkaloids - pharmacology; Antigens, CD - immunology; Biological and medical sciences; Cell Line; Cell physiology; Cytoplasmic Granules - drug effects; Cytoplasmic Granules - physiology; Drug Synergism; Enzyme Activation; Exocytosis - drug effects; Fundamental and applied biological sciences. Psychology; GTP-Binding Proteins - metabolism; Guanosine 5'-O-(3-Thiotriphosphate) - pharmacology; Humans; Killer Cells, Natural - drug effects; Killer Cells, Natural - immunology; Killer Cells, Natural - physiology; Kinetics; Molecular and cellular biology; Protein Kinase C - antagonists & inhibitors; Protein Kinase C - metabolism; Second Messenger Systems; Secretion. Exocytosis; Staurosporine; Tetradecanoylphorbol Acetate - pharmacology; Natural killer cell; Human; Signal transduction; Protein kinase C; Enzyme; Cytotoxicity; Granule; Exocytosis; G protein

The binding of natural killer (NK) cells to either susceptible tumor cells or antibody-coated targets results in rapid activation of phospholipase C (PLC) in NK cells. PLC activation generates inositol-1,4,5-trisphosphate and sn-1,2-diacylglycerol as second messengers, which, in turn, increase intra...