Structures, activity and mechanism of the Type IIS restriction endonuclease PaqCI

• Published in: Nucleic acids research Vol. 51; no. 9; pp. 4467 - 4487
• Main Authors: Kennedy, Madison A, Hosford, Christopher J, Azumaya, Caleigh M, Luyten, Yvette A, Chen, Minyong, Morgan, Richard D, Stoddard, Barry L
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 22.05.2023
• Subjects: BASIC BIOLOGICAL SCIENCES; Biochemistry & Molecular Biology; Deoxyribonucleases, Type II Site-Specific - chemistry; Deoxyribonucleases, Type II Site-Specific - metabolism; DNA - chemistry; DNA - genetics; DNA - metabolism; DNA Breaks, Double-Stranded; Nucleic Acid Enzymes; Substrate Specificity

Abstract Type IIS restriction endonucleases contain separate DNA recognition and catalytic domains and cleave their substrates at well-defined distances outside their target sequences. They are employed in biotechnology for a variety of purposes, including the creation of gene-targeting zinc finger...