A Novel Secreted Protein Toxin from the Insect Pathogenic Bacterium Xenorhabdus nematophila

• Published in: The Journal of biological chemistry Vol. 279; no. 15; pp. 14595 - 14601
• Main Authors: Brown, Susan E., Cao, Anh T., Hines, Eric R., Akhurst, Raymond J., East, Peter D.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 09.04.2004; American Society for Biochemistry and Molecular Biology
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological Assay; Blotting, Western; Cosmids; Dose-Response Relationship, Drug; Electrophoresis, Polyacrylamide Gel; Escherichia coli - metabolism; Galleria mellonella; Gene Library; Helicoverpa armigera; Insecta - microbiology; Larva - microbiology; Molecular Sequence Data; Photorhabdus - metabolism; Recombinant Proteins - chemistry; Software; Time Factors; Toxins, Biological - chemistry; Xenorhabdus - metabolism; Xenorhabdus nematophila

The bacterium Xenorhabdus nematophila is an insect pathogen that produces several proteins that enable it to kill insects. Screening of a cosmid library constructed from X. nematophila strain A24 identified a gene that encoded a novel protein that was toxic to insects. The 42-kDa protein encoded by...