Basic fibroblast growth factor autocrine loop controls human osteosarcoma phenotyping and differentiation

• Published in: Molecular medicine (Cambridge, Mass.) Vol. 8; no. 7; pp. 393 - 404
• Main Authors: Bodo, Maria, Lilli, Cinzia, Bellucci, Catia, Carinci, Paolo, Calvitti, Mario, Pezzetti, Furio, Stabellini, Giordano, Bellocchio, Silvia, Balducci, Chiara, Carinci, Francesco, Baroni, Tiziano
• Format: Journal Article
• Language: English
• Published: England 01.07.2002
• Subjects: Alkaline Phosphatase - genetics; Alkaline Phosphatase - metabolism; Autocrine Communication - drug effects; Bone Neoplasms - metabolism; Bone Neoplasms - pathology; Cell Differentiation - drug effects; Cell Division; Cell Line; Collagen Type I - genetics; Collagen Type I - metabolism; Core Binding Factor Alpha 1 Subunit; Extracellular Matrix - metabolism; Fibroblast Growth Factor 2 - genetics; Fibroblast Growth Factor 2 - metabolism; Fibroblast Growth Factor 2 - pharmacology; Gene Expression Regulation - drug effects; Glycosaminoglycans - biosynthesis; Glycosaminoglycans - genetics; Glycosaminoglycans - metabolism; Humans; Neoplasm Proteins; Osteocalcin - genetics; Osteocalcin - metabolism; Osteosarcoma - metabolism; Osteosarcoma - pathology; Phenotype; Protein Binding; Proteoglycans - genetics; Proteoglycans - metabolism; Receptors, Fibroblast Growth Factor - analysis; Receptors, Fibroblast Growth Factor - genetics; RNA, Messenger - genetics; RNA, Messenger - metabolism; Transcription Factors - genetics; Transcription Factors - metabolism; Tumor Cells, Cultured

We focused on the phenotype of non-mineralizing MG 63 and mineralizing TE 85 human osteosarcoma cells and investigated the role of bFGF in modulating their differentiative responses. Basic FGF expression and bFGF effects on osteocalcin, runt-related transcription factor-2 (RUNX2), matrix molecular p...