H5N1 Vaccine–Elicited Memory B Cells Are Genetically Constrained by the IGHV Locus in the Recognition of a Neutralizing Epitope in the Hemagglutinin Stem

• Published in: The Journal of immunology (1950) Vol. 195; no. 2; pp. 602 - 610
• Main Authors: Wheatley, Adam K, Whittle, James R R, Lingwood, Daniel, Kanekiyo, Masaru, Yassine, Hadi M, Ma, Steven S, Narpala, Sandeep R, Prabhakaran, Madhu S, Matus-Nicodemos, Rodrigo A, Bailer, Robert T, Nabel, Gary J, Graham, Barney S, Ledgerwood, Julie E, Koup, Richard A, McDermott, Adrian B
• Format: Journal Article
• Language: English
• Published: England 15.07.2015
• Subjects: Amino Acid Sequence; Antibodies, Neutralizing - biosynthesis; Antibodies, Neutralizing - blood; Antibodies, Viral - biosynthesis; Antibodies, Viral - blood; Antigens, Viral - chemistry; Antigens, Viral - genetics; Antigens, Viral - immunology; B-Lymphocytes - cytology; B-Lymphocytes - immunology; B-Lymphocytes - virology; Epitopes - chemistry; Epitopes - genetics; Epitopes - immunology; Genetic Loci - immunology; Hemagglutinin Glycoproteins, Influenza Virus - chemistry; Hemagglutinin Glycoproteins, Influenza Virus - genetics; Hemagglutinin Glycoproteins, Influenza Virus - immunology; Humans; Immunologic Memory; Influenza A Virus, H5N1 Subtype - chemistry; Influenza A Virus, H5N1 Subtype - immunology; Influenza Vaccines - administration & dosage; Influenza Vaccines - genetics; Influenza Vaccines - immunology; Influenza, Human - blood; Influenza, Human - immunology; Influenza, Human - prevention & control; Influenza, Human - virology; Molecular Sequence Data; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Recombinant Proteins - immunology; Single-Chain Antibodies - chemistry; Single-Chain Antibodies - genetics; Single-Chain Antibodies - immunology; Vaccination; Vaccines, DNA; Vaccines, Inactivated

Because of significant viral diversity, vaccines that elicit durable and broad protection against influenza have been elusive. Recent research has focused on the potential of highly conserved regions of the viral hemagglutinin (HA) as targets for broadly neutralizing Ab responses. Abs that bind the...