Enhanced cell-surface display of a heterologous protein using SED1 anchoring system in SED1-disrupted Saccharomyces cerevisiae strain

• Published in: Journal of bioscience and bioengineering Vol. 125; no. 3; pp. 306 - 310
• Main Authors: Bamba, Takahiro, Inokuma, Kentaro, Hasunuma, Tomohisa, Kondo, Akihiko
• Format: Journal Article
• Language: English
• Published: Japan Elsevier B.V 01.03.2018
• Subjects: Aspergillus aculeatus; beta-glucosidase; biocatalysts; Cell surface display; cell walls; enzyme activity; ethanol production; ionization; lignocellulose; liquid chromatography; Mass spectrometry; Saccharomyces cerevisiae; SED1; structural proteins; tandem mass spectrometry; yeasts; β-Glucosidase; SED1; β-Glucosidase; Aspergillus aculeatus; Saccharomyces cerevisiae; Cell surface display; Mass spectrometry

Yeast displaying enzymes on the cell surface are used for developing whole-cell biocatalysts. High enzyme activity on the cell surface is required in certain applications such as direct ethanol production from lignocellulosic materials. However, the cell surface enzyme activity is limited by several...