Colorimetric determination of ascorbic acid and the activity of alkaline phosphatase based on the inhibition of the peroxidase-like activity of citric acid-capped Prussian Blue nanocubes

• Published in: Mikrochimica acta (1966) Vol. 186; no. 2; pp. 123 - 7
• Main Authors: Wu, Tengteng, Hou, Wenli, Ma, Zhangyan, Liu, Meiling, Liu, Xiaoying, Zhang, Youyu, Yao, Shouzhuo
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.02.2019; Springer; Springer Nature B.V
• Subjects: Acids; Alkaline phosphatase; Analytical Chemistry; Ascorbic acid; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Citric acid; Colorimetry; Hydrogen peroxide; Hydrolysis; Iron compounds; Microengineering; Nanochemistry; Nanotechnology; Original Paper; Oxidation; Peroxidase; Phosphatase; Phosphatases; Pigments; Vitamin C; 3,3,5,5-Tetramethylbenzidine; Colorimetric assay; Prussian White; Nanocubes; Peroxidase mimetic; Enzyme mimic; Inhibition

Colorimetric methods are described for the determination of ascorbic acid (AA) and alkaline phosphatase (ALP). Both assays are based on the inhibition of the peroxidase (POx)-like activity of Prussian Blue nanocubes (PB NCs) capped with citric acid. They catalyze the oxidation of 3,3,5,5-tetramethyl...