Thiamine Mono- and Diphosphate Phosphatases in Bovine Brain Synaptosomes

• Published in: Biochemistry (Moscow) Vol. 85; no. 3; pp. 378 - 386
• Main Authors: Aleshin, V. A., Mezhenska, O. A., Parkhomenko, Y. M., Kaehne, T., Bunik, V. I.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.03.2020; Springer; Springer Nature B.V
• Subjects: Affinity chromatography; Alkaline phosphatase; Animals; Annotations; Biochemistry; Biomedical and Life Sciences; Biomedicine; Bioorganic Chemistry; Brain; Brain - enzymology; Catalytic Domain; Cattle; CD4 antigen; Chromatography, Affinity; Derivatives; Diphosphates - chemistry; Elution; Enzymes; Exopolyphosphatase; Genome; Genomes; Health aspects; Humans; Hydrogen-Ion Concentration; Life Sciences; Mammals; Mass spectrometry; Mass spectroscopy; Microbiology; Neurodegenerative diseases; Nucleotides; Peptides; pH effects; Phosphatase; Phosphatases; Phosphoric Monoester Hydrolases - chemistry; Proteins; Sodium chloride; Substrate Specificity; Synapses; Synaptosomes; Synaptosomes - enzymology; Thiamine; Thiamine - chemistry; Thiamine diphosphate; Thiamine Monophosphate - chemistry; Thiamine Pyrophosphate - chemistry; Urea; Urea - chemistry; Vitamin B; Vitamin B1; United States; apyrase; DING proteins; HPBP; thiamine; thiamine diphosphatase; thiamine monophosphatase; X-DING-CD4

Neurodegenerative diseases are accompanied by changes in the activity of thiamine mono- and diphosphate phosphatases, but molecular identification of these mammalian enzymes is incomplete. In this work, the protein fraction of bovine brain synaptosomes displaying phosphatase activity toward thiamine...