Molecularly engineered live-attenuated chimeric West Nile/dengue virus vaccines protect rhesus monkeys from West Nile virus

• Published in: Virology (New York, N.Y.) Vol. 314; no. 1; pp. 190 - 195
• Main Authors: Pletnev, Alexander G, Claire, Marisa St, Elkins, Randy, Speicher, Jim, Murphy, Brian R, Chanock, Robert M
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.09.2003
• Subjects: Animals; Antibodies, Viral - blood; Cercopithecus aethiops; Dengue virus; Dengue Virus - genetics; Dengue Virus - immunology; Dengue Virus - metabolism; Disease Models, Animal; Genetic Engineering - methods; Humans; Macaca mulatta; Neutralization Tests; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - immunology; Vaccines, Attenuated - administration & dosage; Vaccines, Attenuated - genetics; Vaccines, Attenuated - immunology; Vaccines, Synthetic - administration & dosage; Vaccines, Synthetic - genetics; Vaccines, Synthetic - immunology; Vero Cells; Viral Envelope Proteins - genetics; Viral Envelope Proteins - immunology; Viral Envelope Proteins - metabolism; Viral Vaccines - administration & dosage; Viral Vaccines - genetics; Viral Vaccines - immunology; West Nile Fever - prevention & control; West Nile virus; West Nile virus - genetics; West Nile virus - immunology; West Nile virus - metabolism

Two molecularly engineered, live-attenuated West Nile virus (WN) vaccine candidates were highly attenuated and protective in rhesus monkeys. The vaccine candidates are chimeric viruses (designated WN/DEN4) bearing the membrane precursor and envelope protein genes of WN on a backbone of dengue 4 viru...