Amino acid losses in ileostomy fluid on a protein-free diet

• Published in: The American journal of clinical nutrition Vol. 59; no. 1; pp. 70 - 73
• Main Authors: Fuller, MF, Milne, A, Harris, CI, Reid, TM, Keenan, R
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Elsevier Inc 01.01.1994; American Society for Clinical Nutrition; American Society for Clinical Nutrition, Inc
• Subjects: ACIDE AMINE; administration & dosage; Amino acid requirements; Amino acids; Amino Acids - metabolism; AMINOACIDOS; AZOTE; BACTERIA; BESOIN NUTRITIONNEL; Biological and medical sciences; Body Fluids; Body Fluids - metabolism; CONTEO DE CELULAS; Diet; DIETA; dietary protein; Dietary Proteins; Dietary Proteins - administration & dosage; DIGESTA; Digestive System; Digestive System - metabolism; endogenous protein; EXCRECION; EXCRETION; EXPERIMENTACION; experimental diets; EXPERIMENTATION; Female; FEMME; FLORA MICROBIANA; FLORE MICROBIENNE; Fluids; HOMBRES; HOMME; Humans; Ileostomy; ileostomy fluid; INTESTIN; intestinal microorganisms; INTESTINOS; Male; Medical sciences; men; metabolism; Middle Aged; MUJERES; NECESIDADES DE NUTRIENTES; nitrogen; NITROGENO; NUMERATION CELLULAIRE; nutrient requirements; obligatory amino acid losses; OPERACIONES QUIRURGICAS; OPERATION CHIRURGICALE; ORINA; plate count; PROTEINAS; PROTEINE; REGIME ALIMENTAIRE; Small intestine; Stomach, duodenum, intestine, rectum, anus; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgery of the digestive system; URINE; women; ileostomy fluid; endogenous protein; obligatory amino acid losses; Amino acid requirements; Human; Ileostomy; Digestive diseases; Aminoacid; Loss
• ISSN: 0002-9165; 1938-3207
• DOI: 10.1093/ajcn/59.1.70

The contribution of losses via the gastrointestinal tract to maintenance amino acid requirements was assessed by collecting the ileostomy fluid of volunteers given a protein-free diet for 5 d. The subjects were eight adult men and women with terminal ileum ileostomies after ulcerative colitis. Four consecutive 24-h collections of both digesta and urine were made. On the last 2 d an antibiotic was given that suppressed microbial activity in the digesta and slightly reduced ileostomy outflow. Mean daily amino acid excretion in ileostomy fluid ranged from 32 mg/d for methionine to ≈ 330 mg/d for aspartate and glutamate. These losses were compared with current international estimates of amino acid requirements. For most essential amino acids gastrointestinal losses accounted for 14–33% of daily maintenance requirements but for threonine the contribution was 61%.