Immunogenicity of a Whole-Cell Pertussis Vaccine with Low Lipopolysaccharide Content in Infants

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Published inClinical and Vaccine Immunology Vol. 16; no. 4; pp. 544 - 550
Main Authors Zorzeto, Tatiane Queiroz, Higashi, Hisako Gondo, da Silva, Marcos Tadeu Nolasco, Carniel, Emilia de Faria, Dias, Waldely Oliveira, Ramalho, Vanessa Domingues, Mazzola, Taís Nitsch, Lima, Simone Corte Batista Souza, Morcillo, André Moreno, Stephano, Marco Antonio, Antonio, Maria Angela Reis de Góes, Zanolli, Maria de Lurdes, Raw, Isaias, Vilela, Maria Marluce dos Santos
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 01.04.2009
American Society for Microbiology (ASM)
Subjects
Antitoxins - blood
Blast
Bordetella pertussis
Bordetella pertussis - immunology
CD3 antigen
CD4 antigen
CD8 antigen
Cell culture
Cell Proliferation
Clinical trials
Cytokines
Cytokines - secretion
Data processing
Enzyme-linked immunosorbent assay
Female
Flow cytometry
g-Interferon
Humans
Immune response
Immunity (cell-mediated)
Immunization, Secondary
Immunogenicity
Immunoglobulin G
Infant
Infants
Interleukin 10
Interleukin 4
Lipopolysaccharides
Lipopolysaccharides - immunology
Lymphocytes B
Lymphocytes T
Male
Peripheral blood mononuclear cells
Pertussis
Pertussis Vaccine - immunology
phytohemagglutinins
T-cell receptor
T-Lymphocyte Subsets - immunology
Toxins
Tumor necrosis factor-a
Vaccine Research
Vaccines
Online AccessGet full text

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Abstract Classifications Services CVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue CVI About CVI Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy CVI RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 1556-6811 Online ISSN: 1556-679X Copyright © 2014 by the American Society for Microbiology.   For an alternate route to CVI .asm.org, visit: CVI       
AbstractList The lack of a clear correlation between the levels of antibody to pertussis antigens and protection against disease lends credence to the possibility that cell-mediated immunity provides primary protection against disease. This phase I comparative trial had the aim of comparing the in vitro cellular immune response and anti-pertussis toxin (anti-PT) immunoglobulin G (IgG) titers induced by a cellular pertussis vaccine with low lipopolysaccharide (LPS) content (wP(low) vaccine) with those induced by the conventional whole-cell pertussis (wP) vaccine. A total of 234 infants were vaccinated at 2, 4, and 6 months with the conventional wP vaccine or the wP(low) vaccine. Proliferation of CD3(+) T cells was evaluated by flow cytometry after 6 days of peripheral blood mononuclear cell culture with stimulation with heat-killed Bordetella pertussis or phytohemagglutinin (PHA). CD3(+), CD4(+), CD8(+), and T-cell receptor gammadelta-positive (gammadelta(+)) cells were identified in the gate of blast lymphocytes. Gamma interferon, tumor necrosis factor alpha, interleukin-4 (IL-4), and IL-10 levels in supernatants and serum anti-PT IgG levels were determined using enzyme-linked immunosorbent assay (ELISA). The net percentage of CD3(+) blasts in cultures with B. pertussis in the group vaccinated with wP was higher than that in the group vaccinated with the wP(low) vaccine (medians of 6.2% for the wP vaccine and 3.9% for the wP(low) vaccine; P = 0.029). The frequencies of proliferating CD4(+), CD8(+), and gammadelta(+) cells, cytokine concentrations in supernatants, and the geometric mean titers of anti-PT IgG were similar for the two vaccination groups. There was a significant difference between the T-cell subpopulations for B. pertussis and PHA cultures, with a higher percentage of gammadelta(+) cells in the B. pertussis cultures (P < 0.001). The overall data did suggest that wP vaccination resulted in modestly better specific CD3(+) cell proliferation, and gammadelta(+) cell expansions were similar with the two vaccines.
The lack of a clear correlation between the levels of antibody to pertussis antigens and protection against disease lends credence to the possibility that cell-mediated immunity provides primary protection against disease. This phase I comparative trial had the aim of comparing the in vitro cellular immune response and anti-pertussis toxin (anti-PT) immunoglobulin G (IgG) titers induced by a cellular pertussis vaccine with low lipopolysaccharide (LPS) content (wP low vaccine) with those induced by the conventional whole-cell pertussis (wP) vaccine. A total of 234 infants were vaccinated at 2, 4, and 6 months with the conventional wP vaccine or the wP low vaccine. Proliferation of CD3 + T cells was evaluated by flow cytometry after 6 days of peripheral blood mononuclear cell culture with stimulation with heat-killed Bordetella pertussis or phytohemagglutinin (PHA). CD3 + , CD4 + , CD8 + , and T-cell receptor γδ-positive (γδ + ) cells were identified in the gate of blast lymphocytes. Gamma interferon, tumor necrosis factor alpha, interleukin-4 (IL-4), and IL-10 levels in supernatants and serum anti-PT IgG levels were determined using enzyme-linked immunosorbent assay (ELISA). The net percentage of CD3 + blasts in cultures with B. pertussis in the group vaccinated with wP was higher than that in the group vaccinated with the wP low vaccine (medians of 6.2% for the wP vaccine and 3.9% for the wP low vaccine; P = 0.029). The frequencies of proliferating CD4 + , CD8 + , and γδ + cells, cytokine concentrations in supernatants, and the geometric mean titers of anti-PT IgG were similar for the two vaccination groups. There was a significant difference between the T-cell subpopulations for B. pertussis and PHA cultures, with a higher percentage of γδ + cells in the B. pertussis cultures ( P < 0.001). The overall data did suggest that wP vaccination resulted in modestly better specific CD3 + cell proliferation, and γδ + cell expansions were similar with the two vaccines.
Classifications Services CVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue CVI About CVI Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy CVI RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 1556-6811 Online ISSN: 1556-679X Copyright © 2014 by the American Society for Microbiology.   For an alternate route to CVI .asm.org, visit: CVI       
ABSTRACT The lack of a clear correlation between the levels of antibody to pertussis antigens and protection against disease lends credence to the possibility that cell-mediated immunity provides primary protection against disease. This phase I comparative trial had the aim of comparing the in vitro cellular immune response and anti-pertussis toxin (anti-PT) immunoglobulin G (IgG) titers induced by a cellular pertussis vaccine with low lipopolysaccharide (LPS) content (wP low vaccine) with those induced by the conventional whole-cell pertussis (wP) vaccine. A total of 234 infants were vaccinated at 2, 4, and 6 months with the conventional wP vaccine or the wP low vaccine. Proliferation of CD3 + T cells was evaluated by flow cytometry after 6 days of peripheral blood mononuclear cell culture with stimulation with heat-killed Bordetella pertussis or phytohemagglutinin (PHA). CD3 + , CD4 + , CD8 + , and T-cell receptor γδ-positive (γδ + ) cells were identified in the gate of blast lymphocytes. Gamma interferon, tumor necrosis factor alpha, interleukin-4 (IL-4), and IL-10 levels in supernatants and serum anti-PT IgG levels were determined using enzyme-linked immunosorbent assay (ELISA). The net percentage of CD3 + blasts in cultures with B. pertussis in the group vaccinated with wP was higher than that in the group vaccinated with the wP low vaccine (medians of 6.2% for the wP vaccine and 3.9% for the wP low vaccine; P = 0.029). The frequencies of proliferating CD4 + , CD8 + , and γδ + cells, cytokine concentrations in supernatants, and the geometric mean titers of anti-PT IgG were similar for the two vaccination groups. There was a significant difference between the T-cell subpopulations for B. pertussis and PHA cultures, with a higher percentage of γδ + cells in the B. pertussis cultures ( P < 0.001). The overall data did suggest that wP vaccination resulted in modestly better specific CD3 + cell proliferation, and γδ + cell expansions were similar with the two vaccines.
The lack of a clear correlation between the levels of antibody to pertussis antigens and protection against disease lends credence to the possibility that cell-mediated immunity provides primary protection against disease. This phase I comparative trial had the aim of comparing the in vitro cellular immune response and anti-pertussis toxin (anti-PT) immunoglobulin G (IgG) titers induced by a cellular pertussis vaccine with low lipopolysaccharide (LPS) content (wPlow vaccine) with those induced by the conventional whole-cell pertussis (wP) vaccine. A total of 234 infants were vaccinated at 2, 4, and 6 months with the conventional wP vaccine or the wPlow vaccine. Proliferation of CD3+ T cells was evaluated by flow cytometry after 6 days of peripheral blood mononuclear cell culture with stimulation with heat-killed Bordetella pertussis or phytohemagglutinin (PHA). CD3+, CD4+, CD8+, and T-cell receptor -positive (+) cells were identified in the gate of blast lymphocytes. Gamma interferon, tumor necrosis factor alpha, interleukin-4 (IL-4), and IL-10 levels in supernatants and serum anti-PT IgG levels were determined using enzyme-linked immunosorbent assay (ELISA). The net percentage of CD3+ blasts in cultures with B. pertussis in the group vaccinated with wP was higher than that in the group vaccinated with the wPlow vaccine (medians of 6.2% for the wP vaccine and 3.9% for the wPlow vaccine; P = 0.029). The frequencies of proliferating CD4+, CD8+, and + cells, cytokine concentrations in supernatants, and the geometric mean titers of anti-PT IgG were similar for the two vaccination groups. There was a significant difference between the T-cell subpopulations for B. pertussis and PHA cultures, with a higher percentage of + cells in the B. pertussis cultures (P < 0.001). The overall data did suggest that wP vaccination resulted in modestly better specific CD3+ cell proliferation, and + cell expansions were similar with the two vaccines.
Author Marco Antonio Stephano
Simone Corte Batista Souza Lima
Marcos Tadeu Nolasco da Silva
Vanessa Domingues Ramalho
Emilia de Faria Carniel
Maria de Lurdes Zanolli
André Moreno Morcillo
Tatiane Queiroz Zorzeto
Waldely Oliveira Dias
Isaias Raw
Maria Marluce dos Santos Vilela
Hisako Gondo Higashi
Maria Ângela Reis de Góes Antonio
Taís Nitsch Mazzola
AuthorAffiliation Center for Investigation in Pediatrics, 1 Pediatrics Department, State University of Campinas Medical School, Rua Tessália Vieira de Camargo 126, Campinas, São Paulo, Brazil CEP 13083-887, 4 Butantan Institute, Rua Vital Brasil, 1500, São Paulo, São Paulo, Brazil CEP 05503-900, 2 Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Av. Prof. Lineu Prestes 580, Butantã, São Paulo, Brazil CEP 05508-000 3
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Corresponding author. Mailing address: Center for Investigation in Pediatrics and Pediatrics Department, State University of Campinas Medical School, Rua Tessália Vieira de Camargo 126, Campinas, São Paulo, Brazil CEP 13083-887. Phone: 55-19-35218963. Fax: 55-19-35218972. E-mail: marluce@fcm.unicamp.br
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SSID ssj0043687
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Snippet Classifications Services CVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit...
The lack of a clear correlation between the levels of antibody to pertussis antigens and protection against disease lends credence to the possibility that...
ABSTRACT The lack of a clear correlation between the levels of antibody to pertussis antigens and protection against disease lends credence to the possibility...
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StartPage 544
SubjectTerms Antitoxins - blood
Blast
Bordetella pertussis
Bordetella pertussis - immunology
CD3 antigen
CD4 antigen
CD8 antigen
Cell culture
Cell Proliferation
Clinical trials
Cytokines
Cytokines - secretion
Data processing
Enzyme-linked immunosorbent assay
Female
Flow cytometry
g-Interferon
Humans
Immune response
Immunity (cell-mediated)
Immunization, Secondary
Immunogenicity
Immunoglobulin G
Infant
Infants
Interleukin 10
Interleukin 4
Lipopolysaccharides
Lipopolysaccharides - immunology
Lymphocytes B
Lymphocytes T
Male
Peripheral blood mononuclear cells
Pertussis
Pertussis Vaccine - immunology
phytohemagglutinins
T-cell receptor
T-Lymphocyte Subsets - immunology
Toxins
Tumor necrosis factor-a
Vaccine Research
Vaccines
Title Immunogenicity of a Whole-Cell Pertussis Vaccine with Low Lipopolysaccharide Content in Infants
URI http://cvi.asm.org/content/16/4/544.abstract
https://www.ncbi.nlm.nih.gov/pubmed/19261771
https://search.proquest.com/docview/21490944
https://search.proquest.com/docview/67127364
https://pubmed.ncbi.nlm.nih.gov/PMC2668281
Volume 16
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