Development of a mreB-targeted real-time PCR method for the quantitative detection of Vibrio harveyi in seawater and biofilm from aquaculture systems

• Published in: Aquaculture Vol. 525; p. 735337
• Main Authors: Mougin, Julia, Roquigny, Roxane, Travers, Marie-Agnès, Grard, Thierry, Bonnin-Jusserand, Maryse, Le Bris, Cédric
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 30.08.2020; Elsevier
• Subjects: Agricultural sciences; antibiotics; Aquaculture; aquaculture systems; Bacteriology; Biofilm; control methods; DNA; fish farms; genes; Gram-negative bacteria; Life Sciences; Microbiology and Parasitology; mreB gene; Quantitative detection; quantitative polymerase chain reaction; sanitizers; Seawater; Vibrio harveyi; Zootechny; Biofilm; mreB gene; Vibrio harveyi; Quantitative detection; Seawater; Aquaculture

Vibrio harveyi is a particularly problematic Gram-negative bacterium because it can form biofilms on aquaculture facility surfaces, leading to resistance of bacteria against antibiotics and water sanitizers. A SYBR Green I quantitative real-time PCR method was developed to detect V. harveyi directly...