Development of a 1-step Enzyme-linked Immunosorbent Assay for the Rapid Diagnosis of Bovine Respiratory Syncytial Virus in Postmortem Specimens

Bovine respiratory syncytial virus (BRSV) is associated with severe respiratory disease in cattle. BRSV infection frequently leads to the death of young infected animals. The presence of BRSV in postmortem specimens is routinely detected using indirect immunofluorescence (IIF). However, this techniq...

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Published inJournal of veterinary diagnostic investigation Vol. 19; no. 3; pp. 238 - 243
Main Authors Quinting, B, Robert, B, Letellier, C, Boxus, M, Kerkhofs, P, Schynts, F, Collard, A
Format Journal Article
LanguageEnglish
Published United States 01.05.2007
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Abstract Bovine respiratory syncytial virus (BRSV) is associated with severe respiratory disease in cattle. BRSV infection frequently leads to the death of young infected animals. The presence of BRSV in postmortem specimens is routinely detected using indirect immunofluorescence (IIF). However, this technique requires special equipment and considerable expertise. The present paper describes the development of a 1-step ELISA for rapid (1.5 hours) detection of BRSV antigen in organ homogenates. The performance of the new 1-step ELISA was evaluated using bovine postmortem specimens (n = 108) in comparison with 3 other BRSV diagnostic techniques: indirect immunofluorescence, the Clearview respiratory syncytial virus (RSV) test, and real-time reverse transcriptase polymerase chain reaction (RT-PCR). The relative sensitivity, specificity, and the kappa coefficient of 1-step ELISA, the Clearview RSV electroimmunoassay (EIA), and IIF were calculated, using real-time RT-PCR as the reference test. The new 1-step ELISA was the most sensitive and specific of the 3 tests. Thus, the new 1-step ELISA is a reliable test for detecting BRSV antigen in organ homogenates.
AbstractList Bovine respiratory syncytial virus (BRSV) is associated with severe respiratory disease in cattle. BRSV infection frequently leads to the death of young infected animals. The presence of BRSV in postmortem specimens is routinely detected using indirect immunofluorescence (IIF). However, this technique requires special equipment and considerable expertise. The present paper describes the development of a 1-step ELISA for rapid (1.5 hours) detection of BRSV antigen in organ homogenates. The performance of the new 1-step ELISA was evaluated using bovine postmortem specimens (n = 108) in comparison with 3 other BRSV diagnostic techniques: indirect immunofluorescence, the Clearview respiratory syncytial virus (RSV) test, and real-time reverse transcriptase polymerase chain reaction (RT-PCR). The relative sensitivity, specificity, and the kappa coefficient of 1-step ELISA, the Clearview RSV electroimmunoassay (EIA), and IIF were calculated, using real-time RT-PCR as the reference test. The new 1-step ELISA was the most sensitive and specific of the 3 tests. Thus, the new 1-step ELISA is a reliable test for detecting BRSV antigen in organ homogenates.
Author Kerkhofs, P
Schynts, F
Collard, A
Quinting, B
Robert, B
Letellier, C
Boxus, M
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BackLink https://www.ncbi.nlm.nih.gov/pubmed/17459851$$D View this record in MEDLINE/PubMed
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Snippet Bovine respiratory syncytial virus (BRSV) is associated with severe respiratory disease in cattle. BRSV infection frequently leads to the death of young...
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SubjectTerms Animals
antigen detection
Antigens, Viral - analysis
Bovine respiratory syncytial virus
Cattle
cattle diseases
Cattle Diseases - diagnosis
Cattle Diseases - virology
disease detection
enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - veterinary
Fluorescent Antibody Technique, Indirect - veterinary
Immunoenzyme Techniques - veterinary
new methods
postmortem changes
rapid methods
Reproducibility of Results
Respiratory Syncytial Virus Infections - diagnosis
Respiratory Syncytial Virus Infections - veterinary
Respiratory Syncytial Virus Infections - virology
Respiratory Syncytial Virus, Bovine - genetics
Respiratory Syncytial Virus, Bovine - isolation & purification
respiratory tract diseases
Respiratory Tract Diseases - diagnosis
Respiratory Tract Diseases - veterinary
Respiratory Tract Diseases - virology
Reverse Transcriptase Polymerase Chain Reaction - veterinary
RNA, Viral - chemistry
RNA, Viral - genetics
Sensitivity and Specificity
viral diseases of animals and humans
Title Development of a 1-step Enzyme-linked Immunosorbent Assay for the Rapid Diagnosis of Bovine Respiratory Syncytial Virus in Postmortem Specimens
URI https://www.ncbi.nlm.nih.gov/pubmed/17459851
Volume 19
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