The Decade of Super-Resolution Microscopy of the Presynapse

The presynaptic compartment of the chemical synapse is a small, yet extremely complex structure. Considering its size, most methods of optical microscopy are not able to resolve its nanoarchitecture and dynamics. Thus, its ultrastructure could only be studied by electron microscopy. In the last deca...

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Published inFrontiers in synaptic neuroscience Vol. 12; p. 32
Main Authors Nosov, Georgii, Kahms, Martin, Klingauf, Jurgen
Format Journal Article
LanguageEnglish
Published Frontiers Media S.A 11.08.2020
Subjects
active zone
CLEM
exo-endocytosis
nanoscopy
Neuroscience
presynapse
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Abstract The presynaptic compartment of the chemical synapse is a small, yet extremely complex structure. Considering its size, most methods of optical microscopy are not able to resolve its nanoarchitecture and dynamics. Thus, its ultrastructure could only be studied by electron microscopy. In the last decade, new methods of optical superresolution microscopy have emerged allowing the study of cellular structures and processes at the nanometer scale. While this is a welcome addition to the experimental arsenal, it has necessitated careful analysis and interpretation to ensure the data obtained remains artifact-free. In this article we review the application of nanoscopic techniques to the study of the synapse and the progress made over the last decade with a particular focus on the presynapse. We find to our surprise that progress has been limited, calling for imaging techniques and probes that allow dense labeling, multiplexing, longer imaging times, higher temporal resolution, while at least maintaining the spatial resolution achieved thus far.
AbstractList The presynaptic compartment of the chemical synapse is a small, yet extremely complex structure. Considering its size, most methods of optical microscopy are not able to resolve its nanoarchitecture and dynamics. Thus, its ultrastructure could only be studied by electron microscopy. In the last decade, new methods of optical superresolution microscopy have emerged allowing the study of cellular structures and processes at the nanometer scale. While this is a welcome addition to the experimental arsenal, it has necessitated careful analysis and interpretation to ensure the data obtained remains artifact-free. In this article we review the application of nanoscopic techniques to the study of the synapse and the progress made over the last decade with a particular focus on the presynapse. We find to our surprise that progress has been limited, calling for imaging techniques and probes that allow dense labeling, multiplexing, longer imaging times, higher temporal resolution, while at least maintaining the spatial resolution achieved thus far.
Author Klingauf, Jurgen
Kahms, Martin
Nosov, Georgii
AuthorAffiliation 2 CIM-IMPRS Graduate Program in Münster , Münster , Germany
1 Institute of Medical Physics and Biophysics, University of Münster , Münster , Germany
AuthorAffiliation_xml – name: 2 CIM-IMPRS Graduate Program in Münster , Münster , Germany
– name: 1 Institute of Medical Physics and Biophysics, University of Münster , Münster , Germany
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  surname: Klingauf
  fullname: Klingauf, Jurgen
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Edited by: Shelley Halpain, University of California, San Diego, United States
Reviewed by: Craig Curtis Garner, Charité – Universitätsmedizin Berlin, Germany; Ling-Gang Wu, National Institute of Neurological Disorders and Stroke (NINDS), United States
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Snippet The presynaptic compartment of the chemical synapse is a small, yet extremely complex structure. Considering its size, most methods of optical microscopy are...
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StartPage 32
SubjectTerms active zone
CLEM
exo-endocytosis
nanoscopy
Neuroscience
presynapse
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Title The Decade of Super-Resolution Microscopy of the Presynapse
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