Genetic Analysis of a Horizontal Resistance Locus BLMR2 in Brassica napus
Leptosphaeria maculans causes blackleg disease in Brassica napus . The blackleg disease is mainly controlled by resistance genes in B. napus . Previous studies have shown that the blackleg resistant BLMR2 locus that conferred horizontal resistance under field conditions, is located on chromosome A10...
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Published in | Frontiers in plant science Vol. 12; p. 663868 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
25.05.2021
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Subjects | |
Online Access | Get full text |
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Summary: | Leptosphaeria maculans
causes blackleg disease in
Brassica napus
. The blackleg disease is mainly controlled by resistance genes in
B. napus
. Previous studies have shown that the blackleg resistant
BLMR2
locus that conferred horizontal resistance under field conditions, is located on chromosome A10 of
B. napus
. The purpose of this study is to fine map this locus and hence identify a candidate gene underlying horizontal resistance. The spectrum of resistance to
L. maculans
isolates of the resistance locus
BLMR2
was analyzed using near isogenic lines, resistant, and susceptible cultivars. The results showed that this locus was horizontally resistant to all isolates tested. Sequence characterized amplified regions (SCAR), simple sequence repeats (SSR), and single nucleotide polymorphism (SNP) markers were developed in the chromosome region of
BLMR2
and a fine genetic map was constructed. Two molecular markers narrowed
BLMR2
in a 53.37 kb region where six genes were annotated. Among the six annotated genes,
BnaA10g11280D/BnaA10g11290D
encoding a cytochrome P450 protein were predicted as the candidate of
BLMR2
. Based on the profiling of pathogen induced transcriptome, three expressed genes in the six annotated genes were identified while only cytochrome
P450
showed upregulation. The candidate corresponds to the gene involved in the indole glucosinolate biosynthesis pathway and plant basal defense in
Arabidopsis thaliana
. The molecular markers identified in this study will allow the quick incorporation of the
BLMR2
allele in rapeseed cultivars to enhance blackleg resistance. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Alexander Idnurm, The University of Melbourne, Australia; Xiaodong Wang, Jiangsu Academy of Agricultural Sciences (JAAS), China Edited by: Jacqueline Batley, University of Western Australia, Australia These authors have contributed equally to this work and share first authorship This article was submitted to Plant Breeding, a section of the journal Frontiers in Plant Science |
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2021.663868 |