Molecular Characterization of the Recombinant A-chain of a Type Ⅱ Ribosome-Inactivating Protein (RIP) from Viscum album coloratum and Structural Basis on its Ribosome-Inactivating Activity and the Sugar-binding Properties of the B-chain

• Published in: BMB reports Vol. 39; no. 5; pp. 560 - 570
• Main Authors: Ye, Wenhui (Nanyang Technological University, Singapore), Nanga, Ravi Prakash Reddy (Nanyang Technological University, Singapore), Kang, C.B. (Nanyang Technological University, Singapore), Song, J.H. (Handong University, Pohang, Republic of Korea), Song, S.K. (Handong University, Pohang, Republic of Korea), Yoon, H.S. (Nanyang Technological University, Singapore), E-mail: hsyoon@ntu.edu.sg
• Format: Journal Article
• Language: English
• Published: Korea (South) 30.09.2006
• Subjects: Amino Acid Sequence; Apoptosis; Carbohydrates - chemistry; Catalytic Domain; Cell Line, Tumor; Cell Survival - drug effects; Circular Dichroism; Glycosylation; Humans; Inhibitory Concentration 50; LECTINAS; LECTINE; LECTINS; Magnetic Resonance Spectroscopy; Models, Molecular; Molecular Sequence Data; N-Glycosyl Hydrolases - metabolism; Plant Preparations - chemistry; Plant Preparations - metabolism; Plant Preparations - pharmacology; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Proteins - pharmacology; Protein Folding; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Recombinant Proteins - pharmacology; Ribosome Inactivating Proteins; Ribosome Inactivating Proteins, Type 2; Ribosome-inactivating protein; Ricin - chemistry; Ricin - genetics; Structural Homology, Protein; Sugar binding; Toxins, Biological - chemistry; Toxins, Biological - genetics; Toxins, Biological - metabolism; Toxins, Biological - pharmacology; Viscum album - chemistry; Viscum album coloratum
• ISSN: 1225-8687; 1976-6696; 0219-1024
• DOI: 10.5483/bmbrep.2006.39.5.560

Mistletoe (Viscum album) lectins, which are classified as a type Ⅱ ribosome-inactivating protein (RIP) due to their unique biological function and the potential medical and therapeutic application in cancer cells, receive a rising attention. The heterodimeric glycoproteins contain the A-chain with catalytic activity and the B-chain with sugar binding properties. In recent years, studies involving the lectins from the white berry European mistletoe (Viscum album) and the yellow berry Korean mistletoe (Viscum album coloratum) have been described.