Epidemiology and Associated Risk Factors for Brucellosis in Small Ruminants Kept at Institutional Livestock Farms in Punjab, Pakistan
Brucellosis is reportedly endemic in ruminants in Pakistan. Both Brucella abortus and B. melitensis infections have been decumented in domestic animals and humans in the country. This study aimed to identify the burden of anti- Brucella antibodies in small ruminants as well as associated potential r...
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Published in | Frontiers in veterinary science Vol. 7; p. 526 |
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Main Authors | , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
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02.09.2020
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Abstract | Brucellosis is reportedly endemic in ruminants in Pakistan. Both
Brucella abortus
and
B. melitensis
infections have been decumented in domestic animals and humans in the country. This study aimed to identify the burden of anti-
Brucella
antibodies in small ruminants as well as associated potential risk factors with its occurrence at nine institutional livestock farms in Punjab, Pakistan. The sera collected from equal number of sheep and goats (500 from each species) were screened by indirect-ELISA for anti-smooth-
Brucella
antibodies followed by a serial detection by real-time PCR. Overall, 5.1% (51/1000) seropositivity was registered corresponding to 5% (25/500) prevalence in goats and 5.2% (26/500) in sheep.
Brucella
-DNA could not be detected in any of the tested sera by real-time PCR. Multiple logistic regression model indicated that farm location (OR 34.05), >4 years of age (OR 2.88), with history of reproductive disorders (OR 2.69), and with BCS of ≤ 3 (OR 12.37) were more likely to test positive for brucellosis at these farms. A routine screening, stringent biosecurity, and quarantine measures are warranted for monitoring and eradication of the infection. Similarly, isolation and molecular investigation of the etiologic agent(s) are needed to understand the relationship of epidemiology and out-breaks of brucellosis in the country. |
---|---|
AbstractList | Brucellosis is reportedly endemic in ruminants in Pakistan. Both
Brucella abortus
and
B. melitensis
infections have been decumented in domestic animals and humans in the country. This study aimed to identify the burden of anti-
Brucella
antibodies in small ruminants as well as associated potential risk factors with its occurrence at nine institutional livestock farms in Punjab, Pakistan. The sera collected from equal number of sheep and goats (500 from each species) were screened by indirect-ELISA for anti-smooth-
Brucella
antibodies followed by a serial detection by real-time PCR. Overall, 5.1% (51/1000) seropositivity was registered corresponding to 5% (25/500) prevalence in goats and 5.2% (26/500) in sheep.
Brucella
-DNA could not be detected in any of the tested sera by real-time PCR. Multiple logistic regression model indicated that farm location (OR 34.05), >4 years of age (OR 2.88), with history of reproductive disorders (OR 2.69), and with BCS of ≤ 3 (OR 12.37) were more likely to test positive for brucellosis at these farms. A routine screening, stringent biosecurity, and quarantine measures are warranted for monitoring and eradication of the infection. Similarly, isolation and molecular investigation of the etiologic agent(s) are needed to understand the relationship of epidemiology and out-breaks of brucellosis in the country. Brucellosis is reportedly endemic in ruminants in Pakistan. Both Brucella abortus and B. melitensis infections have been decumented in domestic animals and humans in the country. This study aimed to identify the burden of anti-Brucella antibodies in small ruminants as well as associated potential risk factors with its occurrence at nine institutional livestock farms in Punjab, Pakistan. The sera collected from equal number of sheep and goats (500 from each species) were screened by indirect-ELISA for anti-smooth-Brucella antibodies followed by a serial detection by real-time PCR. Overall, 5.1% (51/1000) seropositivity was registered corresponding to 5% (25/500) prevalence in goats and 5.2% (26/500) in sheep. Brucella-DNA could not be detected in any of the tested sera by real-time PCR. Multiple logistic regression model indicated that farm location (OR 34.05), >4 years of age (OR 2.88), with history of reproductive disorders (OR 2.69), and with BCS of ≤ 3 (OR 12.37) were more likely to test positive for brucellosis at these farms. A routine screening, stringent biosecurity, and quarantine measures are warranted for monitoring and eradication of the infection. Similarly, isolation and molecular investigation of the etiologic agent(s) are needed to understand the relationship of epidemiology and out-breaks of brucellosis in the country. |
Author | Aslam, Muhammad Aamir Jamil, Huma Jamil, Tariq Qureshi, Zafar Iqbal Schwarz, Stefan Iqbal, Muhammad Amjad Tahir, Usman Neubauer, Heinrich Ullah, Qudrat Hussain, Muhammad Hammad Melzer, Falk Saqib, Muhammad Ullah, Shakeeb |
AuthorAffiliation | 4 Institute of Microbiology and Epizootics, Freie Universität , Berlin , Germany 6 Independent Researcher , Bardia, NSW , Australia 7 Institute of Microbiology, Faculty of Veterinary Science, University of Agriculture , Faisalabad , Pakistan 5 Department of Clinical Medicine and Surgery, Faculty of Veterinary Science, University of Agriculture , Faisalabad , Pakistan 8 Veterinary Research Institute , Lahore , Pakistan 9 Livestock and Dairy Development, Government of Punjab , Lahore , Pakistan 2 Faculty of Veterinary and Animal Sciences, Gomal University , Dera Ismail Khan , Pakistan 1 Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut , Jena , Germany 3 Department of Theriogenology, Faculty of Veterinary Science, University of Agriculture , Faisalabad , Pakistan |
AuthorAffiliation_xml | – name: 1 Institute of Bacterial Infections and Zoonoses, Friedrich-Loeffler-Institut , Jena , Germany – name: 9 Livestock and Dairy Development, Government of Punjab , Lahore , Pakistan – name: 2 Faculty of Veterinary and Animal Sciences, Gomal University , Dera Ismail Khan , Pakistan – name: 8 Veterinary Research Institute , Lahore , Pakistan – name: 3 Department of Theriogenology, Faculty of Veterinary Science, University of Agriculture , Faisalabad , Pakistan – name: 7 Institute of Microbiology, Faculty of Veterinary Science, University of Agriculture , Faisalabad , Pakistan – name: 6 Independent Researcher , Bardia, NSW , Australia – name: 5 Department of Clinical Medicine and Surgery, Faculty of Veterinary Science, University of Agriculture , Faisalabad , Pakistan – name: 4 Institute of Microbiology and Epizootics, Freie Universität , Berlin , Germany |
Author_xml | – sequence: 1 givenname: Qudrat surname: Ullah fullname: Ullah, Qudrat – sequence: 2 givenname: Tariq surname: Jamil fullname: Jamil, Tariq – sequence: 3 givenname: Falk surname: Melzer fullname: Melzer, Falk – sequence: 4 givenname: Muhammad surname: Saqib fullname: Saqib, Muhammad – sequence: 5 givenname: Muhammad Hammad surname: Hussain fullname: Hussain, Muhammad Hammad – sequence: 6 givenname: Muhammad Aamir surname: Aslam fullname: Aslam, Muhammad Aamir – sequence: 7 givenname: Huma surname: Jamil fullname: Jamil, Huma – sequence: 8 givenname: Muhammad Amjad surname: Iqbal fullname: Iqbal, Muhammad Amjad – sequence: 9 givenname: Usman surname: Tahir fullname: Tahir, Usman – sequence: 10 givenname: Shakeeb surname: Ullah fullname: Ullah, Shakeeb – sequence: 11 givenname: Zafar Iqbal surname: Qureshi fullname: Qureshi, Zafar Iqbal – sequence: 12 givenname: Stefan surname: Schwarz fullname: Schwarz, Stefan – sequence: 13 givenname: Heinrich surname: Neubauer fullname: Neubauer, Heinrich |
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CitedBy_id | crossref_primary_10_3389_fpubh_2021_660508 crossref_primary_10_1016_j_rvsc_2023_105048 crossref_primary_10_3390_pathogens9110929 crossref_primary_10_1155_2024_8898827 crossref_primary_10_3390_idr13010018 crossref_primary_10_1016_j_heliyon_2023_e15085 crossref_primary_10_3390_microbiolres14030061 crossref_primary_10_1186_s12917_024_03880_9 crossref_primary_10_3390_ani11061744 crossref_primary_10_1186_s12917_024_03986_0 crossref_primary_10_3389_fvets_2023_1195274 crossref_primary_10_4103_2773_0344_383635 |
Cites_doi | 10.1099/ijs.0.65356-0 10.1099/ijs.0.65269-0 10.20506/rst.32.1.2197 10.3390/ijerph17041412 10.3390/pathogens8040294 10.1007/s40588-017-0052-z 10.1177/104063879500700408 10.3390/ijerph16214271 10.1099/ijsem.0.000998 10.17582/journal.pjz/2019.51.3.sc3 10.1186/s12917-019-1825-9 10.5713/ajas.1995.489 10.1099/ijs.0.011148-0 10.1099/ijs.0.065482-0 10.19045/bspab.2017.60084 10.1089/fpd.2012.1360 10.56808/2985-1130.2843 10.1128/JCM.42.3.1290-1293.2004 10.3390/microorganisms7100449 10.3855/jidc.4993 10.1186/1751-0473-3-17 10.1128/JCM.00837-08 10.14737/journal.jimb/2015/3.2.52.56 |
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Copyright | Copyright © 2020 Ullah, Jamil, Melzer, Saqib, Hussain, Aslam, Jamil, Iqbal, Tahir, Ullah, Qureshi, Schwarz and Neubauer. 2020 Ullah, Jamil, Melzer, Saqib, Hussain, Aslam, Jamil, Iqbal, Tahir, Ullah, Qureshi, Schwarz and Neubauer |
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Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This article was submitted to Veterinary Infectious Diseases, a section of the journal Frontiers in Veterinary Science These authors have contributed equally to this work Reviewed by: Sidharath Dev Thakur, Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya, India; Faham Khamesipour, Shiraz University, Iran Edited by: Anuwat Wiratsudakul, Mahidol University, Thailand |
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Snippet | Brucellosis is reportedly endemic in ruminants in Pakistan. Both
Brucella abortus
and
B. melitensis
infections have been decumented in domestic animals and... Brucellosis is reportedly endemic in ruminants in Pakistan. Both Brucella abortus and B. melitensis infections have been decumented in domestic animals and... |
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StartPage | 526 |
SubjectTerms | brucellosis goats Pakistan risk factors sheep Veterinary Science |
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Title | Epidemiology and Associated Risk Factors for Brucellosis in Small Ruminants Kept at Institutional Livestock Farms in Punjab, Pakistan |
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