Xyloglucan from soybean ( Glycine max) meal is composed of XXXG-type building units

• Published in: Carbohydrate polymers Vol. 42; no. 2; pp. 185 - 191
• Main Authors: Huisman, M.M.H, Weel, K.G.C, Schols, H.A, Voragen, A.G.J
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 2000; Elsevier Science
• Subjects: ALKALI TREATMENT; Applied sciences; Biodegradation; Biological and medical sciences; CELL WALLS; Chelation; COMPOSITION; Composition effects; Enzyme kinetics; Exact sciences and technology; Extraction; Food Chemistry; Food Chemistry Group; Food industries; Fundamental and applied biological sciences. Psychology; GLYCINE (FABACEAE); HEMICELLULOSES; Leerstoelgroep Levensmiddelenchemie; Levensmiddelenchemie; Natural polymers; PARED CELULAR; PAROI CELLULAIRE; pH effects; Physicochemistry of polymers; Plants (botany); POLISACARIDOS; POLYHOLOSIDE; POLYSACCHARIDES; PROCESAMIENTO; PROCESSING; Research and development. New food products, dietetic foods and beverages; SOJA; Solubility; Solutions; Soybean meal; SOYBEANS; Starch and polysaccharides; Sugars; TRAITEMENT; TRAITEMENT AUX ALCALIS; TRATAMIENTO ALCALINO; VLAG; XXXG-type building unit; Xyloglucan; Xyloglucan; XXXG-type building unit; Soybean meal; Glucan; Oside polymer; Molecular structure; Experimental study; Solvent extraction
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/S0144-8617(99)00154-X

Soybean cell wall material was depectinated by extraction with a hot chelating agent and a cold dilute alkali. The hemicelluloses were solubilised from the residue with 1 and 4 M KOH solutions, resulting in 1 M Alkali Soluble Solids (1 MASS) and 4 M Alkali Soluble Solids (4 MASS) fractions. The polysaccharides extracted with 1 M KOH were fractionated by ion-exchange chromatography, yielding a neutral and a pectic population. The sugar composition of the neutral population indicated the presence of xyloglucans and possibly xylans. Enzymatic degradation with endo-xylanases and endo-glucanases showed the presence of xyloglucans only. Analysis of the digest formed after incubation of the neutral population with endo-glucanase V using both HPAEC and MALDI-TOF MS showed the formation of the characteristic poly-XXXG xyloglucan oligomers (XXG, XXXG, XXFG, XLXG, and XLFG).