Structural determinants of specificity and catalytic mechanism in mammalian 25-kDa thiamine triphosphatase

• Published in: Biochimica et biophysica acta Vol. 1830; no. 10; pp. 4513 - 4523
• Main Authors: Delvaux, David, Kerff, Frédéric, Murty, Mamidanna R.V.S., Lakaye, Bernard, Czerniecki, Jan, Kohn, Gregory, Wins, Pierre, Herman, Raphaël, Gabelica, Valérie, Heuze, Fabien, Tordoir, Xavier, Marée, Raphaël, Matagne, André, Charlier, Paulette, De Pauw, Edwin, Bettendorff, Lucien
• Format: Journal Article Web Resource
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2013; Elsevier Science
• Subjects: active sites; Amino Acid Sequence; Animals; Anthozoa; Biocatalysis; Biochemistry, biophysics & molecular biology; Biochimie, biophysique & biologie moléculaire; birds; catalytic activity; chromosomes; Circular Dichroism; crystallography; Crystallography, X-Ray; CyaB, ThTPase; CYTH; Danio rerio; Divalent cation; enzymes; genes; Humans; Life sciences; mammals; mass spectrometry; Models, Molecular; Molecular Docking Simulation; Molecular Sequence Data; Protein Conformation; proteins; Sciences du vivant; Sequence Homology, Amino Acid; Spectrometry, Mass, Electrospray Ionization; Substrate Specificity; thiamin; Thiamin-Triphosphatase - chemistry; Thiamin-Triphosphatase - metabolism; thiamine; Thiamine triphosphate; Triphosphate tunnel metalloenzyme; triphosphate tunnel metalloenzymes; Tripolyphosphate; tryptophan; CD; Thiamine triphosphate; ThDP; ThTPase; Tripolyphosphate; TTM; Triphosphate tunnel metalloenzyme; ThMP; CyaB, ThTPase; PPPase; ThTP; PPPi; Divalent cation; thiamine diphosphate; thiamine triphosphatase; inorganic tripolyphosphate; tripolyphosphatase; PPP(i); circular dichroism; thiamine monophosphate

Thiamine triphosphate (ThTP) is present in most organisms and might be involved in intracellular signaling. In mammalian cells, the cytosolic ThTP level is controlled by a specific thiamine triphosphatase (ThTPase), belonging to the CYTH superfamily of proteins. CYTH proteins are present in all supe...