Separation and study of the range of plasminogen isoforms in patients with prostate cancer
Using affinity chromatography, two-dimensional electrophoresis, and MALDI-TOF mass spectrometry, plasminogen isoforms were separated and identified in blood plasma. Healthy donors and patients with prostate cancer in various stages of development were included in the studied sample. With the develop...
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Published in | Biochemistry (Moscow) Vol. 77; no. 9; pp. 1065 - 1071 |
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Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
SP MAIK Nauka/Interperiodica
01.09.2012
Springer Springer Nature B.V |
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Abstract | Using affinity chromatography, two-dimensional electrophoresis, and MALDI-TOF mass spectrometry, plasminogen isoforms were separated and identified in blood plasma. Healthy donors and patients with prostate cancer in various stages of development were included in the studied sample. With the development of prostate cancer, four additional specific plasminogen isoforms are registered in blood plasma; they are characterized by lower molecular weights and higher p
I
values compared to isoforms found in the control group. |
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AbstractList | Using affinity chromatography, two-dimensional electrophoresis, and MALDI-TOF mass spectrometry, plasminogen isoforms were separated and identified in blood plasma. Healthy donors and patients with prostate cancer in various stages of development were included in the studied sample. With the development of prostate cancer, four additional specific plasminogen isoforms are registered in blood plasma; they are characterized by lower molecular weights and higher pI values compared to isoforms found in the control group.[PUBLICATION ABSTRACT] Using affinity chromatography, two-dimensional electrophoresis, and MALDI-TOF mass spectrometry, plasminogen isoforms were separated and identified in blood plasma. Healthy donors and patients with prostate cancer in various stages of development were included in the studied sample. With the development of prostate cancer, four additional specific plasminogen isoforms are registered in blood plasma; they are characterized by lower molecular weights and higher pI values compared to isoforms found in the control group. Using affinity chromatography, two-dimensional electrophoresis, and MALDI-TOF mass spectrometry, plasminogen isoforms were separated and identified in blood plasma. Healthy donors and patients with prostate cancer in various stages of development were included in the studied sample. With the development of prostate cancer, four additional specific plasminogen isoforms are registered in blood plasma; they are characterized by lower molecular weights and higher pI values compared to isoforms found in the control group. DOI: 10.1134/S0006297912090143 Key words: plasminogen isoforms, two-dimensional electrophoresis, MALDI-TOF-MS, prostate cancer Using affinity chromatography, two-dimensional electrophoresis, and MALDI-TOF mass spectrometry, plasminogen isoforms were separated and identified in blood plasma. Healthy donors and patients with prostate cancer in various stages of development were included in the studied sample. With the development of prostate cancer, four additional specific plasminogen isoforms are registered in blood plasma; they are characterized by lower molecular weights and higher p I values compared to isoforms found in the control group. |
Audience | Academic |
Author | Zimakov, D. V. Kogan, M. I. Belova, T. N. Tarasov, V. A. Boyko, N. V. Chibichian, M. B. Matishov, D. G. Vodolazhsky, D. I. Cherkasova, E. N. Shin, E. F. Moshkovskii, S. A. Golikov, A. Yu |
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Cites_doi | 10.1021/bi970328d 10.1016/S0021-9258(19)86765-0 10.1074/jbc.M205514200 10.1038/nm0596-493 10.1016/0049-3848(85)90224-5 10.1126/science.170.3962.1095 10.1007/s000180300039 10.1007/s00018-010-0566-5 10.1038/nprot.2006.468 10.1016/S0014-5793(97)00221-4 10.1074/jbc.270.11.5877 10.1007/978-0-387-79962-9_4 10.1042/0264-6021:3550397 10.1016/0003-2697(87)90197-7 10.1016/0003-2697(76)90527-3 10.1074/jbc.272.11.7408 10.1016/0168-1176(91)85050-V 10.1007/978-1-4615-0165-7 10.1007/s000180050497 10.1021/ac9806005 10.1016/0167-4838(90)90259-I 10.1038/227680a0 |
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Keywords | two-dimensional electrophoresis MALDI-TOF-MS prostate cancer plasminogen isoforms |
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SubjectTerms | Aged Biochemistry Biomedical and Life Sciences Biomedicine Bioorganic Chemistry Cancer Cancer patients Care and treatment Developmental stages Glycoproteins Humans Life Sciences Male Mass spectrometry Microbiology Middle Aged Oncology, Experimental Plasma Plasminogen - analysis Plasminogen - isolation & purification Prostate cancer Prostatic Neoplasms - blood Proteases Protein Isoforms - blood Proteins Thrombolytic drugs |
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Title | Separation and study of the range of plasminogen isoforms in patients with prostate cancer |
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