Dynamic and transient interactions of Atg9 with autophagosomes, but not membrane integration, are required for autophagy

• Published in: Molecular biology of the cell Vol. 23; no. 10; pp. 1860 - 1873
• Main Authors: Orsi, A, Razi, M, Dooley, H C, Robinson, D, Weston, A E, Collinson, L M, Tooze, S A
• Format: Journal Article
• Language: English
• Published: United States The American Society for Cell Biology 15.05.2012
• Subjects: Animals; Autophagy; Autophagy-Related Protein-1 Homolog; Autophagy-Related Proteins; Biomarkers - metabolism; Carrier Proteins - genetics; Carrier Proteins - metabolism; Gene Knockdown Techniques; HEK293 Cells; Humans; Intracellular Membranes - metabolism; Intracellular Signaling Peptides and Proteins - genetics; Intracellular Signaling Peptides and Proteins - metabolism; Membrane Proteins - genetics; Membrane Proteins - metabolism; Mice; Mice, Knockout; Microscopy, Fluorescence; Microtubule-Associated Proteins - metabolism; Phagosomes - metabolism; Phagosomes - ultrastructure; Phosphate-Binding Proteins; Protein Binding; Protein Serine-Threonine Kinases - genetics; Protein Serine-Threonine Kinases - metabolism; Protein Structure, Tertiary; Protein Transport; Receptors, Transferrin - metabolism; RNA Interference; Vesicular Transport Proteins

Autophagy is a catabolic process essential for cell homeostasis, at the core of which is the formation of double-membrane organelles called autophagosomes. Atg9 is the only known transmembrane protein required for autophagy and is proposed to deliver membrane to the preautophagosome structures and a...