Heterozygous deficiency of manganese superoxide dismutase results in severe lipid peroxidation and spontaneous apoptosis in murine myocardium in vivo

• Published in: Free radical biology & medicine Vol. 38; no. 11; pp. 1458 - 1470
• Main Authors: Strassburger, Maria, Bloch, Wilhelm, Sulyok, Silke, Schüller, Jutta, Keist, Alexander F., Schmidt, Annette, Wenk, Jutta, Peters, Thorsten, Wlaschek, Meinhard, Krieg, Thomas, Hafner, Martin, Kümin, Angelika, Werner, Sabine, Müller, Werner, Scharffetter-Kochanek, Karin
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2005
• Subjects: Animals; Apoptosis; Blotting, Western; Copper, zinc superoxide dismutase; Electrophoresis, Polyacrylamide Gel; Embryo, Mammalian - cytology; Female; Free radicals; Genetic Vectors; Glutathione Peroxidase - metabolism; Heart tissue; Heterozygote; Immunohistochemistry; Integrases - metabolism; Lipid Peroxidation; Male; Manganese superoxide dismutase; Mice; Mice, Inbred C57BL; Mice, Transgenic; Models, Genetic; Mouse model; Myocardium - metabolism; Myocardium - pathology; Oocytes - metabolism; Oxidative Stress; Oxygen - metabolism; Phenotype; Promoter Regions, Genetic; Reactive oxygen species; Recombination, Genetic; Ribonucleases - metabolism; RNA - metabolism; RNA, Messenger - metabolism; Signal Transduction; Stem Cells - cytology; Superoxide anion; Superoxide Dismutase - genetics; Superoxide Dismutase - metabolism; Superoxide Dismutase - physiology; Tyrosine - analogs & derivatives; Tyrosine - chemistry; Tyrosine - metabolism; PBS; Superoxide anion; Reactive oxygen species; Heart tissue; Free radicals; DAB; TBS; PARP; ES cells; SOD2; SOD3; GPx; SOD1; Manganese superoxide dismutase; BSA; ROS; Copper, zinc superoxide dismutase; Mouse model; PHGPx; Apoptosis

To circumvent the early lethality of manganese superoxide dismutase (SOD2)-deficient mice, we have used a skin-specific strategy with introduction of loxP sites flanking exon 3 of the SOD2 gene. To our surprise, when breeding a female keratin 14 Cre transgenic mouse to a SOD2 “floxed” male mouse, du...