Differential protein abundance in promastigotes of nitric oxide-sensitive and resistant Leishmania chagasi strains

• Published in: Proteomics. Clinical applications Vol. 10; no. 11; pp. 1132 - 1146
• Main Authors: Alcolea, Pedro J., Tuñón, Gabriel I. L., Alonso, Ana, García-Tabares, Francisco, Ciordia, Sergio, Mena, María C., Campos, Roseane N. S., Almeida, Roque P., Larraga, Vicente
• Format: Journal Article
• Language: English
• Published: Germany Blackwell Publishing Ltd 01.11.2016; Wiley Subscription Services, Inc
• Subjects: Animals; Arginase; Arginase - genetics; Arginase - metabolism; Bone Marrow - parasitology; Dogs; Drug Resistance; Electrophoresis, Gel, Two-Dimensional; Female; Glucose-6-phosphate dehydrogenase; Glucosephosphate Dehydrogenase - genetics; Glucosephosphate Dehydrogenase - metabolism; Glutathione peroxidase; Glutathione Peroxidase - genetics; Glutathione Peroxidase - metabolism; Leishmania chagasi; Leishmania infantum - drug effects; Leishmania infantum - isolation & purification; Leishmania infantum - metabolism; Leishmaniasis, Visceral - parasitology; Leishmaniasis, Visceral - pathology; Leishmaniasis, Visceral - veterinary; Male; Nitric oxide; Nitric Oxide - toxicity; Nitric oxide resistance; Promastigote; Proteins; Protozoan Proteins - analysis; Protozoan Proteins - genetics; Protozoan Proteins - metabolism; Real-Time Polymerase Chain Reaction; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Glutathione peroxidase; Arginase; Glucose-6-phosphate dehydrogenase; Leishmania chagasi; Nitric oxide resistance; Promastigote

Purpose Leishmania chagasi is the causative agent of zoonotic visceral leishmaniasis in Brazil. Domestic and stray dogs are the main reservoirs. The life cycle of the parasite involves two stages. Promastigotes are extracellular and develop within the sand fly gut. Amastigotes survive inside the har...