Establishment of a novel experimental model for muscle‐invasive bladder cancer using a dog bladder cancer organoid culture

• Published in: Cancer science Vol. 110; no. 9; pp. 2806 - 2821
• Main Authors: Elbadawy, Mohamed, Usui, Tatsuya, Mori, Takashi, Tsunedomi, Ryouichi, Hazama, Shoichi, Nabeta, Rina, Uchide, Tsuyoshi, Fukushima, Ryuji, Yoshida, Toshinori, Shibutani, Makoto, Tanaka, Takaharu, Masuda, Sosuke, Okada, Rena, Ichikawa, Ryo, Omatsu, Tsutomu, Mizutani, Tetsuya, Katayama, Yukie, Noguchi, Shunsuke, Iwai, Satomi, Nakagawa, Takayuki, Shinohara, Yuta, Kaneda, Masahiro, Yamawaki, Hideyuki, Sasaki, Kazuaki
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.09.2019; John Wiley and Sons Inc
• Subjects: Animals; Antineoplastic Agents - pharmacology; Antineoplastic Agents - therapeutic use; Antineoplastic drugs; Antitumor agents; biomarker; Biomarkers; Biomarkers, Tumor - analysis; Biomarkers, Tumor - genetics; Biomarkers, Tumor - metabolism; Bladder cancer; Cancer therapies; Cell adhesion & migration; Cell culture; Cell Culture Techniques - methods; Cell Line, Tumor; Cell Survival - drug effects; Cell viability; Chemotherapy; Cisplatin; Desmoglein 3; dog; Dog Diseases - drug therapy; Dog Diseases - genetics; Dog Diseases - pathology; Dog Diseases - urine; Dogs; Female; Gemcitabine; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Invasiveness; Laboratory animals; Male; Medical prognosis; Metastasis; Neoplasia; organoid; Organoids; Organoids - drug effects; Organoids - metabolism; Organoids - pathology; Original; Piroxicam; Principal components analysis; Prostate cancer; Ribonucleic acid; RNA; RNA‐seq; Sequence Analysis, RNA; Smooth muscle; Stem cells; Tumorigenesis; Up-Regulation; Urinary Bladder - cytology; Urinary Bladder - pathology; Urinary Bladder Neoplasms - drug therapy; Urinary Bladder Neoplasms - pathology; Urinary Bladder Neoplasms - urine; Urinary Bladder Neoplasms - veterinary; Urinary tract; Urine; Urine - cytology; Urothelium - cytology; Veterinary medicine; Vinblastine; Japan; RNA-seq; biomarker; dog; bladder cancer; organoid

In human and dogs, bladder cancer (BC) is the most common neoplasm affecting the urinary tract. Dog BC resembles human muscle‐invasive BC in histopathological characteristics and gene expression profiles, and could be an important research model for this disease. Cancer patient‐derived organoid cult...