Development of an SRM method for absolute quantitation of MYDGF/C19orf10 protein

• Published in: Proteomics. Clinical applications Vol. 10; no. 6; pp. 663 - 670
• Main Authors: Dwivedi, Ravi C., Krokhin, Oleg V., El-Gabalawy, Hani S., Wilkins, John A.
• Format: Journal Article
• Language: English
• Published: Germany Blackwell Publishing Ltd 01.06.2016; Wiley Subscription Services, Inc
• Subjects: Albumins - chemistry; Amino Acid Sequence; Arthritis, Rheumatoid - blood; Arthritis, Rheumatoid - diagnosis; Arthritis, Rheumatoid - pathology; Biological Assay - methods; Biological Assay - standards; C19orf10; Carbon Isotopes; Chemical Precipitation; Humans; Immunoglobulin G - chemistry; Interleukins - blood; Interleukins - isolation & purification; Isotope Labeling - methods; Limit of Detection; Mass Spectrometry - methods; Mass Spectrometry - standards; MYDGF; Nitrogen Isotopes; Peptides; Peptides - analysis; Peptides - chemistry; Recombinant Proteins - biosynthesis; Recombinant Proteins - chemistry; Serum; SRM; Synovial fluid; Synovial Fluid - chemistry; MYDGF; Synovial fluid; Serum; C19orf10; SRM

Purpose To develop a MS‐based selected reaction monitoring (SRM) assay for quantitation of myeloid‐derived growth factor (MYDGF) formerly chromosome 19 open reading frame (C19orf10). Experimental design Candidate reporter peptides were identified in digests of recombinant MYDGF. Isotopically labeled...