The MX4blaster cassette: repeated and clean Saccharomyces cerevisiae genome modification using the genome-wide deletion collection

• Published in: FEMS yeast research Vol. 13; no. 8; pp. 711 - 719
• Main Authors: Carvalho, Ângela, Pereira, Filipa, Johansson, Björn
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.12.2013; Oxford University Press
• Subjects: Base Sequence; clean deletion; counter‐selectable marker; Deletion mutant; Gene Deletion; Gene Expression Regulation, Fungal; Gene Order; Gene Targeting; Genetic Markers; Genetic transformation; Genome, Fungal; Genome-Wide Association Study; Genomes; genome‐wide deletion collection; Molecular Sequence Data; Mutagenesis, Insertional; repeated gene deletion; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - growth & development; Yeast; counter-selectable marker; genome-wide deletion collection; clean deletion; repeated gene deletion; Saccharomyces cerevisiae

Abstract The kanMX4 resistance marker is widely used for Saccharomyces cerevisiae gene deletion and has been used to create a genome-wide deletion mutant collection. Transfer of PCR-amplified marker loci from collection mutants is a very efficient way of introducing mutations into other S. cerevisia...